Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Sediment Sample
2.1.2. Carbon-Free Inorganic Medium
2.1.3. Simulated Viaduct Rainwater
2.1.4. Actual Viaduct Rainwater
2.2. Methods
2.2.1. Isolation and Identification of the Carbon-Fixing Bacteria
- (1)
- Isolation, purification, screening of carbon-fixing bacterial strains
- (2)
- Identification of the carbon-fixing bacterial strain
2.2.2. Optimization of Purification Process of the Carbon-Fixing Bacteria Using the Simulated Viaduct Rainwater
- (1)
- Effect of different simulated rainwater concentrations on the purification efficiency of 1C-1
- (2)
- Effect of different carbon source dosages on the purification efficiency of 1C-1
- (3)
- Effect of different inoculation amounts on the purification effect of 1C-1
2.2.3. The Efficiency of the Carbon-Fixing Bacteria for Actual Viaduct Rainwater Treatment
3. Results and Discussion
3.1. Isolation and Identification of the Carbon-Fixing Bacteria
3.1.1. Morphology Observation of 1C-1
3.1.2. 16S rDNA Analysis of 1C-1
3.2. Optimization of the Purification Process of the Carbon-Fixing Bacteria Using Simulated Viaduct Rainwater
3.2.1. The Effect of Different Simulated Rainwater Concentrations on the Purification Efficiency of 1C-1
3.2.2. The Effect of Different Carbon Source Dosages on the Purification Efficiency of 1C-1
3.2.3. The Effect of Different Inoculation Amounts on the Purification Effect of 1C-1
3.3. The Efficiency of the Carbon-Fixing Bacteria for Actual Viaduct Rainwater Treatment
4. Conclusions
- (1)
- Through isolation, purification, screening, and identification, a carbon-fixing bacteria strain numbered 1C-1 with water purification effects was obtained from the sediment of the viaduct rainwater tank of the bridge in Guangzhou. Based on its morphological characteristics and 16S rDNA gene molecular biology identification, the strain 1C-1 belongs to Streptomyces sp.
- (2)
- Under different simulated viaduct rainwater concentrations, the strain 1C-1 had a relatively high TN removal rate at 1/2 diluent of simulated viaduct rainwater, and a relatively high TP removal rate at 2 times concentrate. To balance the nitrogen and phosphorus removal efficiencies and to ensure practicability, the original concentration was chosen as the optimal simulated viaduct rainwater concentration with removal rates of 29.00%, 50.73%, and 9.55% for NH4+-N, TN, and TP, respectively. Under different carbon source dosages, a glucose dosage of 800 mg showed a relatively high purification efficiency with removal rates of 71.48%, 47.86%, and 10.43% for NH4+-N, TN, and TP, respectively. Under different inoculation amounts, the inoculation amount of 1% showed a relatively high efficiency with removal rates of 58.62%, 58.35%, and 27.32% for NH4+-N, TN, and TP, respectively.
- (3)
- Under the above optimal process conditions of an original concentration of the viaduct rainwater, a carbon source dosage of 800 mg, and an inoculation amount of 1%, the strain 1C-1 removed 92.62%, 6.98%, and 6.16% of NH4+-N, TN, and TP respectively from the actual viaduct rainwater. More interestingly, the removal rates of NH4+-N and TN achieved 43.26% and 78.02%, respectively, for the actual viaduct rainwater, even without carbon source addition.
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bi, S.; Zeng, Q.; Deng, Q.; Liu, H.; Zhou, X.; Xie, C.; Liu, W.; Li, Y. Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater. Water 2023, 15, 2916. https://doi.org/10.3390/w15162916
Bi S, Zeng Q, Deng Q, Liu H, Zhou X, Xie C, Liu W, Li Y. Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater. Water. 2023; 15(16):2916. https://doi.org/10.3390/w15162916
Chicago/Turabian StyleBi, Shiming, Qingjun Zeng, Qianwen Deng, Haohua Liu, Xiaoman Zhou, Chijian Xie, Wen Liu, and Yiyong Li. 2023. "Isolation and Identification of a Carbon-Fixing Bacteria Strain and Its Efficiency for Nitrogen and Phosphorus Removal from Viaduct Rainwater" Water 15, no. 16: 2916. https://doi.org/10.3390/w15162916