Study on the Breeding and Characterization of High-Efficiency Oil-Degrading Bacteria by Mutagenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Isolation and Culture of Petroleum Hydrocarbon Degrading Bacteria
2.3. Mutagenesis Test of Petroleum Hydrocarbon Degrading Bacteria
2.4. Degradation Test of Pollutants by Mutant Strain MXM3U2
2.5. Analysis of Petroleum Hydrocarbons
2.5.1. Determination of the Degradation Proportion of Petroleum Hydrocarbons by UV Spectrophotometry
2.5.2. Determination of the Growth of Petroleum Hydrocarbon-Degrading Bacteria by UV Spectrophotometry
3. Results and Discussion
3.1. Screening and Characteristics of Petroleum Hydrocarbon Degrading Bacteria MX1 Strain
3.2. 16S rDNA Sequence Homology and Phylogenetic Analysis
3.3. Determination of Optimal Growth and Degradation Conditions of the MX1 Strain
3.4. Microwave-UV Mutagenesis of Petroleum Hydrocarbon Degrading Bacteria
3.5. Screening of Mutant Strains by UV and Microwave
3.6. Degradation of Alkanes by Mutant Strain MXM3U2
3.7. Degradation Pathway of Pristane, Naphthalene, and Phenanthrene by Mutant Strains
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Bacteria | Straight Rod-Shaped, 0.6~1.0 μm × 1.2~3.0 μm in Size, Gram-Negative, with Pericytic Flagella. | ||
---|---|---|---|
colony | Round or nearly round, 1–4 mm in diameter, milky white. The surface is slightly moist and shiny, with a slightly raised center and a nearly crenellated edge. | ||
exercise observation | + | Gelatin Liquefaction Test | + |
Anaerobic growth | + | Citrate Utilization Test | + |
aerobic growth | + | Fluorochrome | − |
VP test | + | KCN | + |
indole test | − | contact enzyme | − |
oxidase | + | H2S production test | − |
MR test | − | Sugar fermentation test | + |
methyl red test | + | Urease | − |
Serial Number | t/min | Degradation Products | m/e (%) |
---|---|---|---|
1 | 11.2 | 129 (18.12), 128 (13.43), 101 (12.13), 100 (11.57), 87 (8.19), 69 (12.92), 59 (100.00), 41 (21.08), 39 (10.87) 129 (29.35), 100 (16.58), 59 (100.00), 58 (60.96), 42 (12.87), 41 (21.93) | |
2 | 22.6 | 169 (11.54), 141 (7.09), 129 (9.33), 113 (12.74), 99 (72.95), 88 (100.00), 71 (33.18), 59 (35.37), 43 (41.56) 169, 141, 129, 113, 99, 88, 71, 59, 43 standard from the library NIST | |
3 | 25.7 | 238 (11.35), 226 (7.16), 212 (12.84), 197 (6.39), 185 (24.74), 167 (26.26), 155 (12.54), 125 (4.78), 113 (12.25), 85 (34.73), 71 (11.25), 69 (20.04), 58 (35.79), 43 (100.00) 238, 226, 212, 197, 185, 167, 155, 125, 113, 85, 71, 69, 59, 43 standard from the library NIST | |
4 | 25.4 | 113 (12.43), 99 (10.77), 85 (28.27), 71 (77.91), 69 (11.25), 57 (100.00) 56 (20.62), 55 (20.65), 43 (65.33), 41 (30.34) |
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Zhang, P.; You, Z.; Chen, T.; Zhao, L.; Zhu, J.; Shi, W.; Meng, Q.; Sun, Y. Study on the Breeding and Characterization of High-Efficiency Oil-Degrading Bacteria by Mutagenesis. Water 2022, 14, 2544. https://doi.org/10.3390/w14162544
Zhang P, You Z, Chen T, Zhao L, Zhu J, Shi W, Meng Q, Sun Y. Study on the Breeding and Characterization of High-Efficiency Oil-Degrading Bacteria by Mutagenesis. Water. 2022; 14(16):2544. https://doi.org/10.3390/w14162544
Chicago/Turabian StyleZhang, Pan, Zhaoyang You, Tianfang Chen, Li Zhao, Jianguo Zhu, Weihong Shi, Qinwei Meng, and Yongjun Sun. 2022. "Study on the Breeding and Characterization of High-Efficiency Oil-Degrading Bacteria by Mutagenesis" Water 14, no. 16: 2544. https://doi.org/10.3390/w14162544