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Article

Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater

School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam 690525, India
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Alejandro Gonzalez-Martinez
Water 2022, 14(1), 119; https://doi.org/10.3390/w14010119
Received: 30 November 2021 / Revised: 29 December 2021 / Accepted: 3 January 2022 / Published: 5 January 2022
(This article belongs to the Special Issue Biotechnology Applications in Water and Wastewater Treatment)
Robust control of pathogens in sewage facilitates safe reuse of wastewater rich in valuable nutrients for potential valorization through biological means. Aspergillus niger is widely reported in bioremediation of wastewater but studies on control of enteric pathogens in sewage are very sparse. So, this study aimed at exploring the antibacterial and nematicidal activity of A. niger culture filtrate (ACF). Antibacterial activity of ACF on enteric pathogens (Klebsiella pneumoniae, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella enterica, Shigella dysenteriae, Escherichia coli, Staphylococcus aureus, Klebsiella variicola) was determined by spectrophotometric growth analysis, resazurin based viability assay and biofilm formation assay. ACF showed inhibition against all enteric pathogens except Pseudomonas aeruginosa. Nematicidal studies on Caenorhabditis elegans showed 85% egg hatch inhibition and 52% mortality of L1 larvae. Sewage treatment with ACF at 1:1 (v/v) showed 2–3 log reduction in coliforms, Klebsiella, Shigella, Salmonella, S. aureus and Vibrio except Pseudomonas, indicating significant alteration of complex microbial dynamics in wastewater. Application of ACF can potentially be used as a robust biocontrol strategy against infectious microbes in wastewater and subsequent valorization by cultivating beneficial Pseudomonas. View Full-Text
Keywords: wastewater; Aspergillus niger; enteric pathogens; biocontrol; biofilms wastewater; Aspergillus niger; enteric pathogens; biocontrol; biofilms
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MDPI and ACS Style

Subhash, S.; Babu, P.; Vijayakumar, A.; Suresh, R.A.; Madhavan, A.; Nair, B.G.; Pal, S. Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater. Water 2022, 14, 119. https://doi.org/10.3390/w14010119

AMA Style

Subhash S, Babu P, Vijayakumar A, Suresh RA, Madhavan A, Nair BG, Pal S. Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater. Water. 2022; 14(1):119. https://doi.org/10.3390/w14010119

Chicago/Turabian Style

Subhash, Suja, Pradeesh Babu, Amrutha Vijayakumar, Reshma A. Suresh, Ajith Madhavan, Bipin G. Nair, and Sanjay Pal. 2022. "Aspergillus niger Culture Filtrate (ACF) Mediated Biocontrol of Enteric Pathogens in Wastewater" Water 14, no. 1: 119. https://doi.org/10.3390/w14010119

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