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Article

Effect of the Oxidative Phosphorylation Uncoupler Para-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor

Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67132 Xanthi, Greece
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Academic Editor: Laura Bulgariu
Water 2021, 13(22), 3222; https://doi.org/10.3390/w13223222
Received: 18 October 2021 / Revised: 8 November 2021 / Accepted: 11 November 2021 / Published: 13 November 2021
(This article belongs to the Special Issue Membrane-Based Processes and Applications for Water Reclamation)
In this work, the metabolic uncoupler para-nitrophenol (pNP) was applied to suppress excess sludge production and to investigate its effects on the system’s performance and activated sludge community structure. The COD removal efficiency decreased from 99.0% to 89.5% prior to and after pNP addition, respectively. Application of pNP transiently reduced NH4+-N, NO3-N and NO2-N removal efficiencies, suggesting partial inhibition of both nitrifying and denitrifying activity. However, no changes in the relative abundance of the nitrifying bacteria occurred. Phosphorus removal efficiency was sharply reduced after pNP addition, as the consequence of hydrolysis of stored cell reserves. Tetrasphaera, a key polyphosphate accumulating organism, was also affected by the addition of pNP, a fact that highly influenced system’s ability to remove phosphorus. A drastic drop in Soluble Microbial Products (SMP) and Extracellular Polymeric Substances (EPS) was also detected shortly after the introduction of the uncoupler. On the other hand, MBR’s physicochemical parameters were restored to initial values a week after the addition of pNP. Moreover, remarkable changes in beta-diversity were noted after pNP addition. An increase of Bacteroidetes, Gammaproteobacteria and Firmicutes over Actinobacteria and Alphaproteobacteria was also observed after pNP addition. View Full-Text
Keywords: metabolic uncoupler; nitrophenols; excess sludge; nitrification and denitrification inhibition; Tetrasphaera metabolic uncoupler; nitrophenols; excess sludge; nitrification and denitrification inhibition; Tetrasphaera
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MDPI and ACS Style

Stavrakakis, I.; Remmas, N.; Melidis, P.; Ntougias, S. Effect of the Oxidative Phosphorylation Uncoupler Para-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor. Water 2021, 13, 3222. https://doi.org/10.3390/w13223222

AMA Style

Stavrakakis I, Remmas N, Melidis P, Ntougias S. Effect of the Oxidative Phosphorylation Uncoupler Para-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor. Water. 2021; 13(22):3222. https://doi.org/10.3390/w13223222

Chicago/Turabian Style

Stavrakakis, Ioannis, Nikolaos Remmas, Paraschos Melidis, and Spyridon Ntougias. 2021. "Effect of the Oxidative Phosphorylation Uncoupler Para-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor" Water 13, no. 22: 3222. https://doi.org/10.3390/w13223222

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