Endocrine-disrupting chemicals are highly resistant organic compounds, commonly occurring in the aquatic environment, that can interfere with the endocrine system of animals and humans, causing serious chronic diseases. In recent decades, enzymes from oxidoreductases have been studied for their potential to degrade these compounds effectively. In order to use such enzymes repeatedly, it is necessary to ensure their insolubility in water, a method termed enzyme immobilization. We developed novel polyamide/polyethylenimine (PA/PEI) nanofibers as a promising support material for the immobilization of various biomolecules. Our nanofibers are highly suitable due to a unique combination of mechanical endurance provided by polyamide 6 and their affinity toward biomolecules, ensured by numerous PEI amino groups. Enzyme laccase was successfully immobilized onto PA/PEI nanofibers using a simple and fast method, providing exceptional activity and stability of the attached enzyme. We then tested the degradation ability of the PA/PEI-laccase samples on a highly concentrated mixture of endocrine-disrupting chemicals in real wastewater with adjusted pH. The results indicate that the samples were a suitable material for wastewater treatment by degrading a highly concentrated mixture of bisphenol A, 17α-ethinylestradiol, triclosan, and diclofenac, in real wastewater effluent.
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