Depression of Groundwater Table and Reduced Nitrogen Application Jointly Regulate the Bacterial Composition of nirS-Type and nirK-Type Genes in Agricultural Soil
Abstract
:1. Introduction
2. Materials and Methods
2.1. Field Site and Experimental Description
2.2. Soil Sampling and Physicochemical Properties Analysis
2.3. DNA Extraction, PCR Amplification and Illumina-Based Sequencing
2.4. Amplicon Sequencing and Sequencing Data Processing
2.5. Statistical Analyses
3. Results
3.1. Soil Environmental Parameters
3.2. Soil Bacterial Composition of nirS-Type and nirK-Type Genes
3.3. Influences of Depression of Groundwater Table, Reduced Nitrogen Application and Soil Environmental Parameters on the Bacterial Composition of nirS-Type and nirK-Type Genes
4. Discussion
4.1. Effects of Depression of Groundwater Table and Reduced Nitrogen Application on Soil Attributes
4.2. Effects of Altered Groundwater Table and Reduced Nitrogen Application on Bacterial Composition of Denitrifier Communities
4.3. Influences of Soil Attributes on Bacterial Composition of Denitrifier Communities
4.4. Research Limitation and Future Perspectives
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Source | NO3−–N | NH4+–N | pH | EC | SOC | TSN | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
F | P | F | P | F | P | F | P | F | P | F | P | |
Ninput | 519.10 | <0.001 | 11.27 | <0.01 | 376.15 | <0.001 | 365.49 | <0.001 | 52.65 | <0.001 | 16.12 | <0.001 |
GWD | 121.74 | <0.001 | 4.03 | <0.05 | 172.96 | <0.001 | 18.33 | <0.001 | 44.26 | <0.001 | 39.91 | <0.001 |
Ninput*GWD | 0.22 | 0.80 | 1.53 | 0.24 | 26.73 | <0.001 | 27.90 | <0.001 | 12.85 | <0.001 | 2.75 | 0.09 |
Source | Sinorhizobium | Mesorhizobium | Xanthomonas | Bosea | ||||
---|---|---|---|---|---|---|---|---|
F | P | F | P | F | P | F | P | |
Ninput | 0.12 | 0.74 | 4.51 | <0.05 | 5.02 | <0.05 | 0.08 | 0.78 |
GWD | 32.35 | <0.001 | 25.77 | <0.001 | 1.56 | 0.24 | 11.00 | <0.001 |
Ninput*GWD | 79.60 | <0.001 | 11.83 | <0.001 | 17.58 | <0.001 | 3.88 | <0.05 |
Source | Pseudomonas | Azoarcus | Sulfurifustis | Magnetospirillum | ||||
---|---|---|---|---|---|---|---|---|
F | P | F | P | F | P | F | P | |
Ninput | 4.63 | <0.05 | 5.36 | <0.05 | 0.04 | 0.84 | 6.11 | <0.05 |
GWD | 4.33 | <0.05 | 29.42 | <0.001 | 5.46 | <0.05 | 4.30 | <0.05 |
Ninput*GWD | 0.22 | 0.80 | 15.50 | <0.001 | 2.27 | 0.13 | 0.22 | 0.81 |
Genotype | Ninput | GWD | Ninput*GWD | |
---|---|---|---|---|
nirK-type | F | 1.52 | 1.75 | 1.96 |
P | <0.05 | <0.01 | <0.01 | |
nirS-type | F | 1.61 | 2.10 | 2.11 |
P | <0.05 | <0.01 | <0.01 |
Variables | nirK-Type | nirS-Type | ||
---|---|---|---|---|
r | P | r | P | |
NO3−–N | 0.36 | <0.001 | 0.34 | <0.01 |
NH4+–N | −0.11 | 0.71 | −0.22 | 0.95 |
pH | 0.14 | 0.07 | 0.07 | 0.21 |
EC | 0.27 | <0.01 | 0.32 | <0.01 |
SOC | 0.27 | <0.05 | 0.15 | 0.15 |
TSN | 0.35 | <0.01 | 0.16 | 0.14 |
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Bai, F.; Qi, X.; Li, P.; Qiao, D.; Wang, J.; Du, Z.; She, Y.; Guo, W.; Lu, H. Depression of Groundwater Table and Reduced Nitrogen Application Jointly Regulate the Bacterial Composition of nirS-Type and nirK-Type Genes in Agricultural Soil. Water 2020, 12, 3459. https://doi.org/10.3390/w12123459
Bai F, Qi X, Li P, Qiao D, Wang J, Du Z, She Y, Guo W, Lu H. Depression of Groundwater Table and Reduced Nitrogen Application Jointly Regulate the Bacterial Composition of nirS-Type and nirK-Type Genes in Agricultural Soil. Water. 2020; 12(12):3459. https://doi.org/10.3390/w12123459
Chicago/Turabian StyleBai, Fangfang, Xuebin Qi, Ping Li, Dongmei Qiao, Jianming Wang, Zhenjie Du, Yingjun She, Wei Guo, and Hongfei Lu. 2020. "Depression of Groundwater Table and Reduced Nitrogen Application Jointly Regulate the Bacterial Composition of nirS-Type and nirK-Type Genes in Agricultural Soil" Water 12, no. 12: 3459. https://doi.org/10.3390/w12123459