Characterization of 1,4-Dioxane Biodegradation by a Microbial Community
Abstract
:1. Introduction
2. Materials and Methods
2.1. Enrichment
2.2. Mineralization by Mixed Culture
2.3. Biodegradation at Various Initial Concentrations and Degradation Kinetics of 1,4-dioxane
2.4. Effect of Extra Carbon Sources on 1,4-Dioxane Biodegradation
2.5. Analytical Procedures
3. Results and Discussions
3.1. Mineralization of 1,4-Dioxane
- Cell synthesis from 1,4-dioxane:3C4H8O2 + 5O2 + 2NH3 = 2C5H7NO2 + 2CO2 + 8H2O
- Energy production reaction: (CO2 recovered from 1,4-dioxane)C4H8O2 + 5O2 = 4CO2 + 4H2O
- Biomass decayC5H7NO2 + 5O2 = 5CO2 + NH3 + 2H2O
3.2. Kinetics Parameters Estimation
3.2.1. Endogenous or Decay Rate
3.2.2. Observed Cell Yield
3.2.3. Biodegradation of 1,4-Dioxane with Different Concentrations and Kinetics
3.3. Effects of Ethylene glycol on 1,4-Dioxane Biodegradation
3.4. Effects of THF on 1,4-Dioxane Biodegradation
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Content | Values (mg) | Percentage (%) |
---|---|---|
Cells associated as 1,4-D | 36.4 ± 2.4 | 36.9 ± 6.6 |
CO2 recovered as 1,4-dioxane | 57.5 ± 5.4 | 58.3 ± 9.4 |
Volatile | 3.9 ± 0.1 | 4.0 ± 2.5 |
1,4-dioxane residual in liquid | 0.8 ± 0.1 | 0.8 |
1,4-dioxane | 98.6 | 100 |
1,4-Dioxane Concentration (mg/L) | Degradation Rate (mg/L/h) |
---|---|
5 | 1.793 |
50 | 2.158 |
200 | 3.056 |
500 | 4.375 |
1000 | 6.167 |
1500 | 7.83 |
2000 | 9.125 |
Kinetic Parameters | Value |
---|---|
Ks (mg 1,4-D/L) | 9.42 |
qmax (mg 1,4-D/mg VSS/h) | 0.0063 |
kd (mg VSS/mg VSS/day) | 0.023 |
YT (mg VSS/mg1,4-D) | 0.43 |
μmax (day)−1 | 0.061 |
Kinetic Coefficients | 1,4-Dioxane as Sole Source | THF as Sole Source | 1,4-D&THF 75 mg/L | 1,4-D&THF 150 mg/L |
---|---|---|---|---|
qmax (mg COD/mgVSS/h) | 0.0088 | 0.0167 | 0.0171 | 0.0149 |
Keff (mg/L) | 9.08 | 6.4 | 131.72 | 345.6 |
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Lee, K.H.; Wie, Y.M.; Lee, Y.-S. Characterization of 1,4-Dioxane Biodegradation by a Microbial Community. Water 2020, 12, 3372. https://doi.org/10.3390/w12123372
Lee KH, Wie YM, Lee Y-S. Characterization of 1,4-Dioxane Biodegradation by a Microbial Community. Water. 2020; 12(12):3372. https://doi.org/10.3390/w12123372
Chicago/Turabian StyleLee, Kang Hoon, Young Min Wie, and Yong-Soo Lee. 2020. "Characterization of 1,4-Dioxane Biodegradation by a Microbial Community" Water 12, no. 12: 3372. https://doi.org/10.3390/w12123372