Next Article in Journal
Impact of Relative Sea-Level Rise on Low-Lying Coastal Areas of Catalonia, NW Mediterranean, Spain
Next Article in Special Issue
Photocatalytic Degradation of Polyamide 66; Evaluating the Feasibility of Photocatalysis as a Microfibre-Targeting Technology
Previous Article in Journal
Spatiotemporal Evolution of Evapotranspiration in China after 1998
Previous Article in Special Issue
Towards Characterising Microplastic Abundance, Typology and Retention in Mangrove-Dominated Estuaries
Article

Modification of a Nile Red Staining Method for Microplastics Analysis: A Nile Red Plate Method

1
Department of Land, Water and Environment Research, Korea Institute of Civil Engineering and Building Technology (KICT), Goyang 10223, Korea
2
Civil and Environmental Engineering, University of Science and Technology, Daejeon 34113, Korea
*
Author to whom correspondence should be addressed.
Water 2020, 12(11), 3251; https://doi.org/10.3390/w12113251
Received: 5 October 2020 / Revised: 17 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
Recently, environmental pollution from microplastics (MPs) has become a significant reason for increasing the number of studies to develop analysis methods. The Nile red staining method (NR-S), which is staining polymer particles with Nile red (NR) dye, has been widely used for the analysis of MPs in environmental samples. However, this method has several limitations, as it is difficult to stain MPs covered with organic matter residues. In this study, we modified the NR-S method into an NR plate method (NR-P), where the plate is coated with NR instead of staining MPs directly. The optimum concentration of NR solution was obtained (1000 mg/L), and the effectiveness of the NR-P method for the analysis of MPs was assessed using different types (polypropylene, polyethylene, polyethylene terephthalate, and polystyrene), sizes (100–1000 µm), and shapes (sphere, fiber, film, and flake) of plastic materials. The NR-P method demonstrated improved resolution in the overall types, shapes, and sizes of MPs and was better than the control (without NR plate method) and NR-S method. In particular, the NR-P method can effectively observe MPs covered with organic matter, which was a major limitation of the NR-S method. Finally, MPs in sewage field samples were analyzed by the NR-P method with an accuracy of 78% confirmed by FT-IR. We demonstrated that this method is a convenient and efficient alternative for identifying MPs, even for field samples. View Full-Text
Keywords: microplastics; Nile red staining; Nile red plate; UV light microplastics; Nile red staining; Nile red plate; UV light
Show Figures

Figure 1

MDPI and ACS Style

Kang, H.; Park, S.; Lee, B.; Ahn, J.; Kim, S. Modification of a Nile Red Staining Method for Microplastics Analysis: A Nile Red Plate Method. Water 2020, 12, 3251. https://doi.org/10.3390/w12113251

AMA Style

Kang H, Park S, Lee B, Ahn J, Kim S. Modification of a Nile Red Staining Method for Microplastics Analysis: A Nile Red Plate Method. Water. 2020; 12(11):3251. https://doi.org/10.3390/w12113251

Chicago/Turabian Style

Kang, Heejun; Park, Saerom; Lee, Bokjin; Ahn, Jaehwan; Kim, Seogku. 2020. "Modification of a Nile Red Staining Method for Microplastics Analysis: A Nile Red Plate Method" Water 12, no. 11: 3251. https://doi.org/10.3390/w12113251

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop