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Decoding the Heart through Next Generation Sequencing Approaches

1
International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland
2
Max-Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Genes 2018, 9(6), 289; https://doi.org/10.3390/genes9060289
Received: 28 April 2018 / Revised: 28 May 2018 / Accepted: 4 June 2018 / Published: 7 June 2018
(This article belongs to the Special Issue Emerging Applications for Next Generation Sequencing)
Vertebrate organs develop through a complex process which involves interaction between multiple signaling pathways at the molecular, cell, and tissue levels. Heart development is an example of such complex process which, when disrupted, results in congenital heart disease (CHD). This complexity necessitates a holistic approach which allows the visualization of genome-wide interaction networks, as opposed to assessment of limited subsets of factors. Genomics offers a powerful solution to address the problem of biological complexity by enabling the observation of molecular processes at a genome-wide scale. The emergence of next generation sequencing (NGS) technology has facilitated the expansion of genomics, increasing its output capacity and applicability in various biological disciplines. The application of NGS in various aspects of heart biology has resulted in new discoveries, generating novel insights into this field of study. Here we review the contributions of NGS technology into the understanding of heart development and its disruption reflected in CHD and discuss how emerging NGS based methodologies can contribute to the further understanding of heart repair. View Full-Text
Keywords: next generation sequencing; genomics; epigenetics; whole exome sequencing; RNA-seq; ChIP-seq; heart development; congenital heart disease; heart regeneration; GWAS next generation sequencing; genomics; epigenetics; whole exome sequencing; RNA-seq; ChIP-seq; heart development; congenital heart disease; heart regeneration; GWAS
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Pawlak, M.; Niescierowicz, K.; Winata, C.L. Decoding the Heart through Next Generation Sequencing Approaches. Genes 2018, 9, 289.

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