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Genes 2018, 9(10), 493;

Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1)

Computational Biology Group, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Veterinary Biosciences, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3052, Australia
Department of Biology; Microbiology and Archaea, TU Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
Department of Microbiology, Immunobiology and Genetics, MFPL Laboratories, University of Vienna, Dr. Bohr-Gasse 9, Vienna 1030, Austria
Author to whom correspondence should be addressed.
Received: 11 September 2018 / Revised: 5 October 2018 / Accepted: 8 October 2018 / Published: 12 October 2018
(This article belongs to the Special Issue Genetics of Halophilic Microorganisms)
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The halophilic myohalovirus Halobacterium virus phiH (ΦH) was first described in 1982 and was isolated from a spontaneously lysed culture of Halobacterium salinarum strain R1. Until 1994, it was used extensively as a model to study the molecular genetics of haloarchaea, but only parts of the viral genome were sequenced during this period. Using Sanger sequencing combined with high-coverage Illumina sequencing, the full genome sequence of the major variant (phiH1) of this halovirus has been determined. The dsDNA genome is 58,072 bp in length and carries 97 protein-coding genes. We have integrated this information with the previously described transcription mapping data. PhiH could be classified into Myoviridae Type1, Cluster 4 based on capsid assembly and structural proteins (VIRFAM). The closest relative was Natrialba virus phiCh1 (φCh1), which shared 63% nucleotide identity and displayed a high level of gene synteny. This close relationship was supported by phylogenetic tree reconstructions. The complete sequence of this historically important virus will allow its inclusion in studies of comparative genomics and virus diversity. View Full-Text
Keywords: halovirus; virus; halophage; Halobacterium salinarum; Archaea; haloarchaea; halobacteria; genome inversion halovirus; virus; halophage; Halobacterium salinarum; Archaea; haloarchaea; halobacteria; genome inversion

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Dyall-Smith, M.; Pfeifer, F.; Witte, A.; Oesterhelt, D.; Pfeiffer, F. Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1). Genes 2018, 9, 493.

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