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Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues

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National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, Italy
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Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Sicily, Italy
*
Authors to whom correspondence should be addressed.
Genes 2018, 9(1), 30; https://doi.org/10.3390/genes9010030
Received: 10 November 2017 / Revised: 5 January 2018 / Accepted: 5 January 2018 / Published: 11 January 2018
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses. View Full-Text
Keywords: cnidarians; transcriptome wide analysis; translationally controlled tumour protein; TCTPs; comparative genomics; homology modelling; gene expression cnidarians; transcriptome wide analysis; translationally controlled tumour protein; TCTPs; comparative genomics; homology modelling; gene expression
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Nicosia, A.; Bennici, C.; Biondo, G.; Costa, S.; Di Natale, M.; Masullo, T.; Monastero, C.; Ragusa, M.A.; Tagliavia, M.; Cuttitta, A. Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues. Genes 2018, 9, 30.

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