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Genes 2017, 8(8), 200;

Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis

College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
Author to whom correspondence should be addressed.
Received: 7 July 2017 / Revised: 4 August 2017 / Accepted: 7 August 2017 / Published: 11 August 2017
(This article belongs to the Section Plant Genetics and Genomics)
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Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp. According to annotation version 10.0 of the genome of Arabidopsis thaliana, there are 127,854 introns in the nuclear genes; 99.23% of them are less than 1 kb, and only 16 introns are annotated to be larger than 5 kb, which are extremely large introns (ELI) in Arabidopsis. To learn whether these introns are true introns or not and how large introns could be in Arabidopsis, RT-PCR analysis of genes containing these ELIs were carried out. The results indicated that some of these putative introns are indeed ELIs. These ELIs are mainly composed of transposons or transposable elements (TE), excepting one, whose counterparts are also very long in diverse plant species. Thus, this study confirms the existence of introns larger than 5 kb or even 10 kb in Arabidopsis. View Full-Text
Keywords: Arabidopsis; large intron; transposon; reverse transcription PCR Arabidopsis; large intron; transposon; reverse transcription PCR

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Chang, N.; Sun, Q.; Hu, J.; An, C.; Gao, H. Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis. Genes 2017, 8, 200.

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