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Genes 2017, 8(7), 172;

Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)

Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922, Korea
Department of Fisheries Science, Chonnam National University, 50, Daehak-ro, Yeosu, Jeonnam 59626, Korea
Author to whom correspondence should be addressed.
Academic Editors: Sarvajeet S. Gill, Narendra Tuteja, Ritu Gill, Juan Francisco Jimenez Bremont, Anca Macovei and Naser A. Anjum.
Received: 4 May 2017 / Revised: 8 June 2017 / Accepted: 21 June 2017 / Published: 27 June 2017
(This article belongs to the Special Issue Genetic Regulation of Abiotic Stress Responses)
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Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars. View Full-Text
Keywords: abiotic stress; Botrytis inoculation; stress-related genes; RACE-PCR; Lilium formolongi abiotic stress; Botrytis inoculation; stress-related genes; RACE-PCR; Lilium formolongi

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Howlader, J.; Park, J.-I.; Robin, A.H.K.; Sumi, K.R.; Nou, I.-S. Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi). Genes 2017, 8, 172.

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