Next Article in Journal
Maintenance of Genome Integrity: How Mammalian Cells Orchestrate Genome Duplication by Coordinating Replicative and Specialized DNA Polymerases
Next Article in Special Issue
The Complex Relationship between Virulence and Antibiotic Resistance
Previous Article in Journal
Type 2 Diabetes Susceptibility in the Greek-Cypriot Population: Replication of Associations with TCF7L2, FTO, HHEX, SLC30A8 and IGF2BP2 Polymorphisms
Previous Article in Special Issue
Quorum Sensing Regulation of Competence and Bacteriocins in Streptococcus pneumoniae and mutans
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Genes 2017, 8(1), 23;

Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
Infectiologie et Santé Publique ISP, Institut National de la Recherche Agronomique, Université de Tours, Equipe Bactéries et Risque Materno-fœtal, UMR1282 Tours, France
Author to whom correspondence should be addressed.
Academic Editor: Helen J. Wing
Received: 7 October 2016 / Revised: 29 December 2016 / Accepted: 4 January 2017 / Published: 6 January 2017
(This article belongs to the Special Issue Virulence Gene Regulation in Bacteria)
PDF [2183 KB, uploaded 6 January 2017]


Streptococcus agalactiae (group B Streptococcus; GBS) is a common inhabitant of the genitourinary and/or gastrointestinal tract in up to 40% of healthy adults; however, this opportunistic pathogen is able to breach restrictive host barriers to cause disease and persist in harsh and changing conditions. This study sought to identify a role for quorum sensing, a form of cell to cell communication, in the regulation of the fibrinogen-binding (rgfBDAC) two-component system and the ability to associate with decidualized endometrial cells in vitro. To do this, we created a deletion in rgfD, which encodes the putative autoinducing peptide, in a GBS strain belonging to multilocus sequence type (ST)-17 and made comparisons to the wild type. Sequence variation in the rgf operon was detected in 40 clinical strains and a non-synonymous single nucleotide polymorphism was detected in rgfD in all of the ST-17 genomes that resulted in a truncation. Using qPCR, expression of rgf operon genes was significantly decreased in the ST-17 ΔrgfD mutant during exponential growth with the biggest difference (3.3-fold) occurring at higher cell densities. Association with decidualized endometrial cells was decreased 1.3-fold in the mutant relative to the wild type and rgfC expression was reduced 22-fold in ΔrgfD following exposure to the endometrial cells. Collectively, these data suggest that this putative quorum sensing molecule is important for attachment to human tissues and demonstrate a role for RgfD in GBS pathogenesis through regulation of rgfC. View Full-Text
Keywords: Streptococcus agalactiae; quorum sensing; colonization; group B Streptococcus Streptococcus agalactiae; quorum sensing; colonization; group B Streptococcus

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Parker, R.E.; Knupp, D.; Al Safadi, R.; Rosenau, A.; Manning, S.D. Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus. Genes 2017, 8, 23.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top