A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Plasmids
2.2. Growth Media and Conditions
2.3. Fluorescence Microscopy
2.4. Total RNA Extraction
2.5. Global Transcription Microarray Analysis
2.6. Relative Quantitative Real-Time PCR
2.7. Immunoprecipitation by GFP-Trap
2.8. SDS-PAGE and Mass Spectrometry Analysis
2.9. Bacterial Two Hybrid Analysis
2.10. Site-Directed Mutagenesis
3. Results
3.1. The TorR Protein Is Localized at the Old Cell Pole as a Functional Focus
3.2. TorR Regulates Transcription of Several Genes in a Cell-Cycle-Dependent Manner
3.3. TorR Interacts with MreB and DnaK for Targeting the Old Cell Poles
3.4. CCCP Treatment Affects the Polar Localization of TorR and Expression of the Target Genes
3.5. FtsZ Affects the TorR Foci Formation
3.6. Interaction of TorR with MreB or DnaK is Responsible for Its Polar Localization and Subsequent Regulatory Effect on Gene Expression
4. Discussion
4.1. The TorR Protein Targets the Old Cell Poles by Interacting with MreB and DnaK Proteins
4.2. A Spatial Control for Correct Timing of Gene Expression during the Cell Cycle
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yao, Y.; Fan, L.; Shi, Y.; Odsbu, I.; Morigen. A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle. Genes 2017, 8, 1. https://doi.org/10.3390/genes8010001
Yao Y, Fan L, Shi Y, Odsbu I, Morigen. A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle. Genes. 2017; 8(1):1. https://doi.org/10.3390/genes8010001
Chicago/Turabian StyleYao, Yuan, Lifei Fan, Yixin Shi, Ingvild Odsbu, and Morigen. 2017. "A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle" Genes 8, no. 1: 1. https://doi.org/10.3390/genes8010001