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In Vivo RNAi-Based Screens: Studies in Model Organisms
Department of Life Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan
* Author to whom correspondence should be addressed.
Received: 29 September 2013; in revised form: 29 October 2013 / Accepted: 14 November 2013 / Published: 25 November 2013
Abstract: RNA interference (RNAi) is a technique widely used for gene silencing in organisms and cultured cells, and depends on sequence homology between double-stranded RNA (dsRNA) and target mRNA molecules. Numerous cell-based genome-wide screens have successfully identified novel genes involved in various biological processes, including signal transduction, cell viability/death, and cell morphology. However, cell-based screens cannot address cellular processes such as development, behavior, and immunity. Drosophila and Caenorhabditis elegans are two model organisms whose whole bodies and individual body parts have been subjected to RNAi-based genome-wide screening. Moreover, Drosophila RNAi allows the manipulation of gene function in a spatiotemporal manner when it is implemented using the Gal4/UAS system. Using this inducible RNAi technique, various large-scale screens have been performed in Drosophila, demonstrating that the method is straightforward and valuable. However, accumulated results reveal that the results of RNAi-based screens have relatively high levels of error, such as false positives and negatives. Here, we review in vivo RNAi screens in Drosophila and the methods that could be used to remove ambiguity from screening results.
Keywords: Drosophila; genome-wide screen; RNAi library; false results; interaction network
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Yamamoto-Hino, M.; Goto, S. In Vivo RNAi-Based Screens: Studies in Model Organisms. Genes 2013, 4, 646-665.
Yamamoto-Hino M, Goto S. In Vivo RNAi-Based Screens: Studies in Model Organisms. Genes. 2013; 4(4):646-665.
Yamamoto-Hino, Miki; Goto, Satoshi. 2013. "In Vivo RNAi-Based Screens: Studies in Model Organisms." Genes 4, no. 4: 646-665.