Heritability of Radiation Response in Lung Cancer Families
Abstract
:1. Introduction
2. Results and Discussion
Generations | Composition of family | Total | |||||
---|---|---|---|---|---|---|---|
Parents | LC case | Siblings | Partners | Offspring | |||
1 (unsuitable for the estimation of heritability) | -- | 1 | -- | -- | -- | 1 | 9 |
-- | 1 | -- | ≥1 | -- | 1 | ||
-- | 1 | ≥1 | ≥1 | -- | 7 | ||
2 | -- | -- | ≥1 | ≥1 | ≥1 | 1 | 133 |
-- | 1 | -- | -- | ≥1 | 4 | ||
-- | 1 | -- | ≥1 | ≥1 | 9 | ||
-- | 1 | ≥1 | ≥1 | ≥1 | 31 | ||
1 | -- | -- | ≥1 | -- | 1 | ||
1 | 1 | -- | -- | -- | 6 | ||
1 | 1 | -- | ≥1 | -- | 35 | ||
1 | 1 | ≥1 | ≥1 | -- | 3 | ||
2 | 1 | -- | -- | -- | 18 | ||
2 | 1 | -- | ≥1 | -- | 24 | ||
2 | 1 | ≥1 | -- | -- | 1 | ||
3 | 1 | 1 | -- | -- | ≥1 | 1 | 35 |
1 | 1 | -- | ≥1 | ≥1 | 10 | ||
1 | 1 | ≥1 | -- | ≥1 | 1 | ||
1 | 1 | ≥1 | ≥1 | ≥1 | 9 | ||
2 | 1 | -- | -- | ≥1 | 1 | ||
2 | 1 | -- | ≥1 | ≥1 | 3 | ||
2 | 1 | ≥1 | -- | ≥1 | 3 | ||
2 | 1 | ≥1 | ≥1 | ≥1 | 7 | ||
Total | 177 |
2.1. DNA Damage and DNA Repair
2.2. Impact of Age, Sex and Smoking
2.3. Heritability
Falsity-correction
| Nominal heritability
| Corrected heritability
| |
---|---|---|---|
pseudo-families | LC-families | LC-families | |
Basal damage | 23% | 77% (63%–91% ) | 70% (52%–88%) |
Initial damage | 28% | 75%(62%–88% ) | 65% (47%–83%) |
Repair 10 min | 22% | 56% (45%–67% ) | 44% (29%–58%) |
Repair 30 min | 18% | 34% (24%–43% ) | 20% (07%–30%) |
Repair 60 min | 4% | 50% (36%–65% ) | 48% (33%–64%) |
3. Experimental Section
3.1. Study Groups
3.2. Isolation of Lymphocytes, Irradiation and DNA Repair
3.3. Exposure Level and Batch Processing, COMET Assay, Image Acquisition and COMET Analysis
3.4. Measures for DNA Damage
3.5. Impact of Age, Sex and Smoking
3.6. Estimation of Heritability
4. Conclusions
Acknowledgments
Conflict of interest
References
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Rosenberger, A.; Rössler, U.; Hornhardt, S.; Sauter, W.; Bickeböller, H.; Wichmann, H.-E.; Gomolka, M. Heritability of Radiation Response in Lung Cancer Families. Genes 2012, 3, 248-260. https://doi.org/10.3390/genes3020248
Rosenberger A, Rössler U, Hornhardt S, Sauter W, Bickeböller H, Wichmann H-E, Gomolka M. Heritability of Radiation Response in Lung Cancer Families. Genes. 2012; 3(2):248-260. https://doi.org/10.3390/genes3020248
Chicago/Turabian StyleRosenberger, Albert, Ute Rössler, Sabine Hornhardt, Wiebke Sauter, Heike Bickeböller, H.-Erich Wichmann, and Maria Gomolka. 2012. "Heritability of Radiation Response in Lung Cancer Families" Genes 3, no. 2: 248-260. https://doi.org/10.3390/genes3020248
APA StyleRosenberger, A., Rössler, U., Hornhardt, S., Sauter, W., Bickeböller, H., Wichmann, H.-E., & Gomolka, M. (2012). Heritability of Radiation Response in Lung Cancer Families. Genes, 3(2), 248-260. https://doi.org/10.3390/genes3020248