Next Article in Journal
The Evolution of Protein Structures and Structural Ensembles Under Functional Constraint
Next Article in Special Issue
Occurrence of Plasmids in the Aromatic Degrading Bacterioplankton of the Baltic Sea
Previous Article in Journal
Involvement of Type IV Pili in Pathogenicity of Plant Pathogenic Bacteria
Article Menu

Export Article

Open AccessArticle

Proteomics Analysis of the Effects of Cyanate on Chromobacterium violaceum Metabolism

Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará 66075-110, Brasil
REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica 2829-516, Portugal
Author to whom correspondence should be addressed.
Genes 2011, 2(4), 736-747;
Received: 15 August 2011 / Revised: 26 September 2011 / Accepted: 28 September 2011 / Published: 19 October 2011
PDF [860 KB, uploaded 19 October 2011]


Chromobacterium violaceum is a gram-negative betaproteobacterium that has been isolated from various Brazilian ecosystems. Its genome contains the cyn operon, which gives it the ability to metabolize highly toxic cyanate into ammonium and carbon dioxide. We used a proteomics approach to investigate the effects of cyanate on the metabolism of this bacterium. The proteome of cells grown with and without cyanate was compared on 2-D gels. Differential spots were digested and identified by mass spectrometry. The bacterium was able to grow at concentrations of up to 1 mM cyanate. Eighteen spots were differentially expressed in the presence of cyanate, of which 16 were downregulated and only two were upregulated. An additional 12 spots were detected only in extracts of cells unexposed to cyanate, and one was expressed only by the exposed cells. Fourteen spots were identified, corresponding to 13 different proteins. We conclude that cyanate promotes expression of enzymes that combat oxidative stress and represses enzymes of the citric acid cycle, strongly affecting the energetic metabolism of the cell. Other proteins that were under-expressed in bacteria exposed to cyanate are involved in amino-acid metabolism or are hypothetical proteins, demonstrating that cyanate also affects expression of genes that are not part of the cyn operon. View Full-Text
Keywords: 2DE; bacteria; cyanate; mass spectrometry; proteomic 2DE; bacteria; cyanate; mass spectrometry; proteomic
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Baraúna, R.A.; Ciprandi, A.; Santos, A.V.; Carepo, M.S.; Gonçalves, E.C.; Schneider, M.P.; Silva, A. Proteomics Analysis of the Effects of Cyanate on Chromobacterium violaceum Metabolism. Genes 2011, 2, 736-747.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top