TERTmonitor—qPCR Detection of TERTp Mutations in Glioma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tumour Samples
2.2. Genotyping Characterisation
2.2.1. DNA Extraction
2.2.2. Amplification and Sanger Sequencing
2.2.3. TERTmonitor
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sanger Sequencing, n (%) | TERTmonitor, n (%) | |||||
---|---|---|---|---|---|---|
−124 G>A | −146 G>A | Total | −124 G>A | −146 G>A | Total | |
Pilocytic astrocytoma (n = 8) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Diffuse astrocytoma (n = 29) | 4 (13.8) | 0 (0.0) | 4 (13.8) | 4 (13.8) | 1 (3.4) | 5 (17.2) |
Oligodendroglioma (n = 29) | 11 (37.9) | 5 (17.2) | 16 (55.2) | 11 (37.9) | 5 (17.2) | 16 (55.2) |
Anaplastic oligodendroglioma (n = 37) | 14 (37.8) | 9 (24.3) | 23 (62.2) | 14 (37.8) | 9 (24.3) | 23 (62.2) |
Glioblastoma (n = 76) | 40 (52.6) | 12 (15.8) | 52 (68.4) | 40 (52.6) | 13 (17.1) | 53 (69.7) |
Gliosarcoma (n = 6) | 3 (50.0) | 3 (50.0) | 6 (100.0) | 3 (50.0) | 3 (50.0) | 6 (100.0) |
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Brás, J.P.; Jesus, T.T.; Prazeres, H.; Lima, J.; Soares, P.; Vinagre, J. TERTmonitor—qPCR Detection of TERTp Mutations in Glioma. Genes 2023, 14, 1693. https://doi.org/10.3390/genes14091693
Brás JP, Jesus TT, Prazeres H, Lima J, Soares P, Vinagre J. TERTmonitor—qPCR Detection of TERTp Mutations in Glioma. Genes. 2023; 14(9):1693. https://doi.org/10.3390/genes14091693
Chicago/Turabian StyleBrás, João Paulo, Tito Teles Jesus, Hugo Prazeres, Jorge Lima, Paula Soares, and João Vinagre. 2023. "TERTmonitor—qPCR Detection of TERTp Mutations in Glioma" Genes 14, no. 9: 1693. https://doi.org/10.3390/genes14091693