phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Husbandry and Genetic Lines
2.2. Genotyping Mutant Lines
2.3. In Situ Hybridization and Correlation with Genotype
2.4. Morpholino Injection
2.5. Cloning and Expression of phox2ba-sfGFP Fusion
2.6. Selection of Anesthetic Concentration
2.7. Opercular Beat Analysis
2.8. Heart Rate Analysis
2.9. Statistical Analysis
3. Results
3.1. phox2ba Expression Is Reduced in chd7 Mutants
3.2. Morpholino-Mediated Knockdown of phox2ba Mimics the Decreased Heart Rates Observed in chd7−/− Larvae
3.3. Chd7 Mutant Adults Exhibit Differential Response to Anesthesia and Opercular Rates
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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MacLean, J.E.; Wertman, J.N.; Prykhozhij, S.V.; Chedrawe, E.; Langley, S.; Steele, S.L.; Ban, K.; Blake, K.; Berman, J.N. phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes. Genes 2023, 14, 1086. https://doi.org/10.3390/genes14051086
MacLean JE, Wertman JN, Prykhozhij SV, Chedrawe E, Langley S, Steele SL, Ban K, Blake K, Berman JN. phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes. Genes. 2023; 14(5):1086. https://doi.org/10.3390/genes14051086
Chicago/Turabian StyleMacLean, Jessica E., Jaime N. Wertman, Sergey V. Prykhozhij, Emily Chedrawe, Stewart Langley, Shelby L. Steele, Kevin Ban, Kim Blake, and Jason N. Berman. 2023. "phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes" Genes 14, no. 5: 1086. https://doi.org/10.3390/genes14051086