Development of Improved DNA Collection and Extraction Methods for Handled Documents
Abstract
:1. Introduction
2. Materials and Methods
2.1. Dry/Dry Flocked Swab Technique
2.2. Wet/Dry Flocked Swab Technique
2.3. Direct Cutting Technique
2.4. Vacuum Swab Technique
2.4.1. Glass Pasteur Pipette
2.4.2. Plastic Pipette Tip
2.5. Extraction, Quantitation, Amplification
2.6. Stability Study
3. Results
3.1. Comparison of Sampling Technique
3.2. Vacuum Swab Technique
3.3. Stability Study
4. Discussion
4.1. Direct Cutting and Swabbing Study
4.2. Vacuum Swab Study
4.3. Stability Study
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Glass | Plastic | |||
---|---|---|---|---|
Vac Dry | Vac Wet | Vac Dry | Vac Wet | |
Chelex-Tween | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
0.0004 | 0.0006 | 0.0021 | 0.003 | |
0.0002 | 0.0016 | 0.0062 | 0.0039 |
Month | Donor 001C (ng/µL) | Donor 002C (ng/µL) | Donor 005C (ng/µL) |
---|---|---|---|
0 | 0.0019 | 0.0644 | 0.0003 |
1 | 0.0007 | 0.0183 | 0.0003 |
2 | 0.0033 | 3.2568 | 0.0014 |
3 | 0.0037 | 0.0248 | 0 |
4 | 0.0011 | 0.0301 | 0.001 |
5 | 0.0033 | 0.0861 | 0.0005 |
6 | 0.001 | 0.1125 | 0.0009 |
7 | 0.0002 | 0.0854 | 0.0017 |
8 | 0.0009 | 0.0413 | 0.0028 |
9 | 0.0021 | 0.0296 | 0.002 |
10 | 0.0012 | 0.0454 | 0.003 |
11 | 0.0009 | 0.0135 | 0.0007 |
Average | 0.0016 | 0.0501 | 0.0012 |
Standard Deviation | 0.0011 | 0.0324 | 0.0009 |
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Morgan, A.G.; Prinz, M. Development of Improved DNA Collection and Extraction Methods for Handled Documents. Genes 2023, 14, 761. https://doi.org/10.3390/genes14030761
Morgan AG, Prinz M. Development of Improved DNA Collection and Extraction Methods for Handled Documents. Genes. 2023; 14(3):761. https://doi.org/10.3390/genes14030761
Chicago/Turabian StyleMorgan, Ashley G., and Mechthild Prinz. 2023. "Development of Improved DNA Collection and Extraction Methods for Handled Documents" Genes 14, no. 3: 761. https://doi.org/10.3390/genes14030761