Next Article in Journal
The Role of ACE, ACE2, and AGTR2 Polymorphisms in COVID-19 Severity and the Presence of COVID-19-Related Retinopathy
Previous Article in Journal
Identification of COVID-19-Associated DNA Methylation Variations by Integrating Methylation Array and scRNA-Seq Data at Cell-Type Resolution
Previous Article in Special Issue
Genetic Diversity and Population Structure of Fusarium commune Causing Strawberry Root Rot in Southcentral China
 
 
Article

Genetic Analyses of Discrete Geographic Samples of a Golden Chanterelle in Canada Reveal Evidence for Recent Regional Differentiation

1
Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
2
College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
3
Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: Isabel Henriques
Genes 2022, 13(7), 1110; https://doi.org/10.3390/genes13071110
Received: 20 May 2022 / Revised: 16 June 2022 / Accepted: 20 June 2022 / Published: 21 June 2022
(This article belongs to the Special Issue Population Genetics of Fungi)
The wild edible mushroom Cantharellus enelensis is a recently described species of the golden chanterelles found in eastern North America. At present, the genetic diversity and population structure of C. enelensis are not known. In this study, we analyzed a total of 230 fruiting bodies of C. enelensis that were collected from three regions of Canada: near the east and west coasts of Newfoundland (NFLD), with 110 fruiting bodies each, and around Hamilton, Ontario (10 fruiting bodies). Among the 110 fruiting bodies from each coast in NFLD, 10 from 2009 were without specific site information, while 100 sampled in 2010 were from each of five patches separated by at least 100 m from each other. Each fruiting body was genotyped at three microsatellite loci. Among the total 28 multilocus genotypes (MLGs) identified, 2 were shared among all three regions, 4 were shared between 2 of the 3 regions, and the remaining 22 were each found in only 1 region. Minimal spanning network analyses revealed several region-specific MLG clusters, consistent with geographic specific mutation and expansion. Though the most frequently observed MLGs were shared among local (patch) and regional populations, population genetic analyses revealed that both local and regional geographic separations contributed significantly to the observed genetic variation in the total sample. All three regional populations showed excess heterozygosity; for the eastern NFLD population, we reject the null hypothesis of Hardy–Weinberg equilibrium (HWE) at all three loci. However, the analyses of clone-corrected samples revealed that most loci were in HWE. Together, our results suggest that the three discrete regional populations of C. enelensis were likely colonized from a common refugium since the last ice age. However, the local and regional populations are diverging from each other through mutation, drift, and selection at least partly due to heterozygous advantage. View Full-Text
Keywords: edible mushroom; microsatellite genotyping; population genetics; range expansion; Hardy–Weinberg equilibrium; excess heterozygotes edible mushroom; microsatellite genotyping; population genetics; range expansion; Hardy–Weinberg equilibrium; excess heterozygotes
Show Figures

Graphical abstract

MDPI and ACS Style

Zhao, K.; Korfanty, G.A.; Xu, J.; Thorn, R.G. Genetic Analyses of Discrete Geographic Samples of a Golden Chanterelle in Canada Reveal Evidence for Recent Regional Differentiation. Genes 2022, 13, 1110. https://doi.org/10.3390/genes13071110

AMA Style

Zhao K, Korfanty GA, Xu J, Thorn RG. Genetic Analyses of Discrete Geographic Samples of a Golden Chanterelle in Canada Reveal Evidence for Recent Regional Differentiation. Genes. 2022; 13(7):1110. https://doi.org/10.3390/genes13071110

Chicago/Turabian Style

Zhao, Kuan, Gregory A. Korfanty, Jianping Xu, and R. Greg Thorn. 2022. "Genetic Analyses of Discrete Geographic Samples of a Golden Chanterelle in Canada Reveal Evidence for Recent Regional Differentiation" Genes 13, no. 7: 1110. https://doi.org/10.3390/genes13071110

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop