Next Article in Journal
Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications
Previous Article in Journal
Metformin Triggers Apoptosis and Induction of the G0/G1 Switch 2 Gene in Macrophages
Article

Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Jae-Heung Ko
Genes 2021, 12(9), 1435; https://doi.org/10.3390/genes12091435
Received: 17 August 2021 / Revised: 8 September 2021 / Accepted: 14 September 2021 / Published: 17 September 2021
(This article belongs to the Section Plant Genetics and Genomics)
Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for Larix kaempferi (Japanese larch). Here, we analyzed the expression pattern of L. kaempferi cyclin-dependent kinase B 1;2 (LaCDKB1;2) during somatic embryogenesis in L. kaempferi and its relationship with the cell proliferation rate. We also analyzed the effect of LaCDKB1;2 over-expression on somatic embryo quality. The results revealed a positive correlation between LaCDKB1;2 expression and the cell proliferation rate during the proliferation stage. After LaCDKB1;2 over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that LaCDKB1;2 not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new Larix breeding material. View Full-Text
Keywords: Larix kaempferi; LaCDKB1; 2; cell-cycle gene; somatic embryogenesis Larix kaempferi; LaCDKB1; 2; cell-cycle gene; somatic embryogenesis
Show Figures

Figure 1

MDPI and ACS Style

Kang, Y.; Li, W.; Zhang, L.; Qi, L. Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis. Genes 2021, 12, 1435. https://doi.org/10.3390/genes12091435

AMA Style

Kang Y, Li W, Zhang L, Qi L. Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis. Genes. 2021; 12(9):1435. https://doi.org/10.3390/genes12091435

Chicago/Turabian Style

Kang, Yanhui, Wanfeng Li, Lifeng Zhang, and Liwang Qi. 2021. "Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis" Genes 12, no. 9: 1435. https://doi.org/10.3390/genes12091435

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop