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Article

The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula

1
Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 6708 PB Wageningen, The Netherlands
2
Bioinformatics Group, Department of Plant Sciences, Wageningen University, 6708 PB Wageningen, The Netherlands
*
Author to whom correspondence should be addressed.
Current address: Center for Desert Agriculture, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Current address: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829 Cologne, Germany.
Academic Editor: Attila Kereszt
Genes 2021, 12(7), 988; https://doi.org/10.3390/genes12070988
Received: 15 May 2021 / Revised: 23 June 2021 / Accepted: 24 June 2021 / Published: 28 June 2021
Nitrogen fixation by rhizobia is a highly energy-demanding process. Therefore, nodule initiation in legumes is tightly regulated. Environmental nitrate is a potent inhibitor of nodulation. However, the precise mechanism by which this agent (co)regulates the inhibition of nodulation is not fully understood. Here, we demonstrate that in Medicago truncatula the lipo-chitooligosaccharide-induced accumulation of cytokinins is reduced in response to the application of exogenous nitrate. Under permissive nitrate conditions, perception of rhizobia-secreted signalling molecules leads to an increase in the level of four cytokinins (i.e., iP, iPR, tZ, and tZR). However, under high-nitrate conditions, this increase in cytokinins is reduced. The ethylene-insensitive mutant Mtein2/sickle, as well as wild-type plants grown in the presence of the ethylene biosynthesis inhibitor 2-aminoethoxyvinyl glycine (AVG), is resistant to the inhibition of nodulation by nitrate. This demonstrates that ethylene biosynthesis and perception are required to inhibit nodule organogenesis under high-nitrate conditions. View Full-Text
Keywords: Medicago truncatula; nitrate; nodulation; ethylene; cytokinin Medicago truncatula; nitrate; nodulation; ethylene; cytokinin
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MDPI and ACS Style

Gühl, K.; Holmer, R.; Xiao, T.T.; Shen, D.; Wardhani, T.A.K.; Geurts, R.; van Zeijl, A.; Kohlen, W. The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula. Genes 2021, 12, 988. https://doi.org/10.3390/genes12070988

AMA Style

Gühl K, Holmer R, Xiao TT, Shen D, Wardhani TAK, Geurts R, van Zeijl A, Kohlen W. The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula. Genes. 2021; 12(7):988. https://doi.org/10.3390/genes12070988

Chicago/Turabian Style

Gühl, Kerstin, Rens Holmer, Ting T. Xiao, Defeng Shen, Titis A.K. Wardhani, René Geurts, Arjan van Zeijl, and Wouter Kohlen. 2021. "The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula" Genes 12, no. 7: 988. https://doi.org/10.3390/genes12070988

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