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Genome Features of Asaia sp. W12 Isolated from the Mosquito Anopheles stephensi Reveal Symbiotic Traits

1
Department of Clinical and Diagnostic Sciences, School of Health Sciences, Oakland University, 433 Meadowbrook Road, Rochester, MI 48309, USA
2
Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
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Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université (AMU), UMR 7257, 13288 Marseille, France
4
Institut National de la Recherche Agronomique (INRA), USC AFMB, 1408 Marseille, France
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Department of Biological Sciences, King Abdulaziz University, Jeddah 21412, Saudi Arabia
6
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Helen J. Wing
Genes 2021, 12(5), 752; https://doi.org/10.3390/genes12050752
Received: 24 January 2021 / Revised: 4 May 2021 / Accepted: 6 May 2021 / Published: 17 May 2021
(This article belongs to the Section Microbial Genetics and Genomics)
Asaia bacteria commonly comprise part of the microbiome of many mosquito species in the genera Anopheles and Aedes, including important vectors of infectious agents. Their close association with multiple organs and tissues of their mosquito hosts enhances the potential for paratransgenesis for the delivery of antimalaria or antivirus effectors. The molecular mechanisms involved in the interactions between Asaia and mosquito hosts, as well as Asaia and other bacterial members of the mosquito microbiome, remain underexplored. Here, we determined the genome sequence of Asaia strain W12 isolated from Anopheles stephensi mosquitoes, compared it to other Asaia species associated with plants or insects, and investigated the properties of the bacteria relevant to their symbiosis with mosquitoes. The assembled genome of strain W12 had a size of 3.94 MB, the largest among Asaia spp. studied so far. At least 3585 coding sequences were predicted. Insect-associated Asaia carried more glycoside hydrolase (GH)-encoding genes than those isolated from plants, showing their high plant biomass-degrading capacity in the insect gut. W12 had the most predicted regulatory protein components comparatively among the selected Asaia, indicating its capacity to adapt to frequent environmental changes in the mosquito gut. Two complete operons encoding cytochrome bo3-type ubiquinol terminal oxidases (cyoABCD-1 and cyoABCD-2) were found in most Asaia genomes, possibly offering alternative terminal oxidases and allowing the flexible transition of respiratory pathways. Genes involved in the production of 2,3-butandiol and inositol have been found in Asaia sp. W12, possibly contributing to biofilm formation and stress tolerance. View Full-Text
Keywords: Asaia; paratransgenesis; symbiotic traits; Anopheles stephensi; genome features Asaia; paratransgenesis; symbiotic traits; Anopheles stephensi; genome features
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MDPI and ACS Style

Chen, S.; Yu, T.; Terrapon, N.; Henrissat, B.; Walker, E.D. Genome Features of Asaia sp. W12 Isolated from the Mosquito Anopheles stephensi Reveal Symbiotic Traits. Genes 2021, 12, 752. https://doi.org/10.3390/genes12050752

AMA Style

Chen S, Yu T, Terrapon N, Henrissat B, Walker ED. Genome Features of Asaia sp. W12 Isolated from the Mosquito Anopheles stephensi Reveal Symbiotic Traits. Genes. 2021; 12(5):752. https://doi.org/10.3390/genes12050752

Chicago/Turabian Style

Chen, Shicheng, Ting Yu, Nicolas Terrapon, Bernard Henrissat, and Edward D. Walker 2021. "Genome Features of Asaia sp. W12 Isolated from the Mosquito Anopheles stephensi Reveal Symbiotic Traits" Genes 12, no. 5: 752. https://doi.org/10.3390/genes12050752

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