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Detection of Quantitative Trait Loci (QTL) Associated with the Fruit Morphology of Tomato
Article

Discovery of a Major QTL Controlling Trichome IV Density in Tomato Using K-Seq Genotyping

1
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, COMAV, Universitat Politècnica de València, 46022 Valencia, Spain
2
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, 46980 Paterna, Spain
3
Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA 30602, USA
4
Department of Horticulture, University of Georgia, Athens, GA 30602, USA
*
Author to whom correspondence should be addressed.
Both authors contributed equally.
Academic Editor: Christian Chevalier
Genes 2021, 12(2), 243; https://doi.org/10.3390/genes12020243
Received: 18 January 2021 / Revised: 2 February 2021 / Accepted: 4 February 2021 / Published: 8 February 2021
(This article belongs to the Special Issue Genetic Improvement for Horticultural Plants)
Trichomes are a common morphological defense against pests, in particular, type IV glandular trichomes have been associated with resistance against different invertebrates. Cultivated tomatoes usually lack or have a very low density of type IV trichomes. Therefore, for sustainable management of this crop, breeding programs could incorporate some natural defense mechanisms, such as those afforded by trichomes, present in certain Solanum species. We have identified a S. pimpinellifolium accession with very high density of this type of trichomes. This accession was crossed with a S. lycopersicum var. cerasiforme and a S. lycopersicum var. lycopersicum accessions, and the two resulting F2 populations have been characterized and genotyped using a new genotyping methodology, K-seq. We have been able to build an ultra-dense genetic map with 147,326 SNP markers with an average distance between markers of 0.2 cm that has allowed us to perform a detailed mapping. We have used two different families and two different approaches, QTL mapping and QTL-seq, to identify several QTLs implicated in the control of trichome type IV developed in this accession on the chromosomes 5, 6, 9 and 11. The QTL located on chromosome 9 is a major QTL that has not been previously reported in S. pimpinellifolium. This QTL could be easily introgressed in cultivated tomato due to the close genetic relationship between both species. View Full-Text
Keywords: trichomes type IV; trichomes; tomato; QTL; K-seq; Solanum pimpinellifolium trichomes type IV; trichomes; tomato; QTL; K-seq; Solanum pimpinellifolium
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MDPI and ACS Style

Mata-Nicolás, E.; Montero-Pau, J.; Gimeno-Paez, E.; García-Pérez, A.; Ziarsolo, P.; Blanca, J.; van der Knaap, E.; Díez, M.J.; Cañizares, J. Discovery of a Major QTL Controlling Trichome IV Density in Tomato Using K-Seq Genotyping. Genes 2021, 12, 243. https://doi.org/10.3390/genes12020243

AMA Style

Mata-Nicolás E, Montero-Pau J, Gimeno-Paez E, García-Pérez A, Ziarsolo P, Blanca J, van der Knaap E, Díez MJ, Cañizares J. Discovery of a Major QTL Controlling Trichome IV Density in Tomato Using K-Seq Genotyping. Genes. 2021; 12(2):243. https://doi.org/10.3390/genes12020243

Chicago/Turabian Style

Mata-Nicolás, Estefanía, Javier Montero-Pau, Esther Gimeno-Paez, Ana García-Pérez, Peio Ziarsolo, José Blanca, Esther van der Knaap, María J. Díez, and Joaquín Cañizares. 2021. "Discovery of a Major QTL Controlling Trichome IV Density in Tomato Using K-Seq Genotyping" Genes 12, no. 2: 243. https://doi.org/10.3390/genes12020243

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