Transcriptomic Markers of Recombinant Human Erythropoietin Micro-Dosing in Thoroughbred Horses
Abstract
:1. Introduction
2. Material and Methods
2.1. Horses
2.2. Sample Collection
2.3. PBMC and RNA Isolations
2.4. RNA Sequencing and Analysis
2.5. RT-q PCR Validation
3. Results
3.1. Horses
3.2. RNA Sequencing and Differential Transcript Analysis
3.3. RT-qPCR
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time Point | Gene Name | Log2FC | Ave Expr | p Value | adj. p. Val |
---|---|---|---|---|---|
D7 v D0 | BCORL1 | −6.91 | 3.58 | 1.04 × 10−6 | 7.90 × 10−3 |
C13H16orf54 | 6.68 | 3.98 | 1.48 × 10−6 | 7.90 × 10−3 | |
LOC100630352 | 6.32 | 5.13 | 2.25 × 10−6 | 8.04 × 10−3 | |
SLC16A13 | −6.61 | 4.45 | 7.80 × 10−6 | 1.53 × 10-2 | |
ARHGEF3 | −7.18 | 3.39 | 1.06 × 10−5 | 1.53 × 10−2 | |
LOC111776172 | −4.96 | 5.93 | 1.11 × 10−5 | 1.53 × 10−2 | |
LOC106783439 | 4.70 | 5.05 | 1.14 × 10−5 | 1.53 × 10−2 | |
LOC111767722 | −6.38 | 2.94 | 1.15 × 10−5 | 1.53 × 10-2 | |
PUM2 | 4.40 | 5.19 | 1.28 × 10−5 | 1.53 × 10−2 | |
CCDC6 | −6.17 | 2.92 | 1.43 × 10−5 | 1.53 × 10−2 | |
INTS10 | −6.56 | 2.85 | 1.61 × 10−5 | 1.57 × 10−2 | |
SAV1 | −6.48 | 3.80 | 2.74 × 10−5 | 2.45 × 10−2 | |
RBBP7 | −5.70 | 4.16 | 3.41 × 10−5 | 2.80 × 10−2 | |
ACVR1B | −6.41 | 3.24 | 3.66 × 10−5 | 2.80 × 10-2 | |
CHTOP | −4.03 | 4.68 | 4.42 × 10−5 | 3.15 × 10−2 | |
NSG1 | 5.34 | 3.92 | 7.67 × 10−5 | 4.98 × 10−2 | |
ZBTB14 | −6.47 | 1.41 | 7.91 × 10−5 | 4.98 × 10−2 | |
D7 v D3 | LOC106783439 | 5.31 | 5.05 | 2.76 × 10−6 | 2.42 × 10−3 |
CHTOP | −5.00 | 4.68 | 4.53 × 10−7 | 2.42 × 10−3 | |
PUM2 | 4.11 | 5.19 | 1.21 × 10−5 | 4.32 × 10-2 | |
C13H16orf54 | 5.55 | 3.98 | 2.08 × 10−5 | 4.32 × 10−2 | |
SLC20A1 | −5.43 | 3.42 | 2.17 × 10−5 | 4.32 × 10−2 | |
D28 v D0 | SNX32 | −0.39 | 13.85 | 9.48 × 10−7 | 1.01 × 10−2 |
D28 v D7 | LOC106783439 | −5.08 | 5.05 | 2.90 × 10−7 | 3.10 × 10−3 |
LOC100066849 | 7.49 | 2.30 | 1.32 × 10−6 | 7.08 × 10−3 | |
WBP11 | 5.08 | 5.08 | 3.27 × 10−6 | 1.07 × 10−2 | |
CHTOP | 4.24 | 4.68 | 1.25 × 10−5 | 3.35 × 10−2 | |
D49 v D7 | LOC106783439 | −5.90 | 5.05 | 7.55 × 10−9 | 8.08 × 10−5 |
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Dahlgren, A.R.; Knych, H.K.; Arthur, R.M.; Durbin-Johnson, B.P.; Finno, C.J. Transcriptomic Markers of Recombinant Human Erythropoietin Micro-Dosing in Thoroughbred Horses. Genes 2021, 12, 1874. https://doi.org/10.3390/genes12121874
Dahlgren AR, Knych HK, Arthur RM, Durbin-Johnson BP, Finno CJ. Transcriptomic Markers of Recombinant Human Erythropoietin Micro-Dosing in Thoroughbred Horses. Genes. 2021; 12(12):1874. https://doi.org/10.3390/genes12121874
Chicago/Turabian StyleDahlgren, Anna R., Heather K. Knych, Rick M. Arthur, Blythe P. Durbin-Johnson, and Carrie J. Finno. 2021. "Transcriptomic Markers of Recombinant Human Erythropoietin Micro-Dosing in Thoroughbred Horses" Genes 12, no. 12: 1874. https://doi.org/10.3390/genes12121874