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Article

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

1
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 676, Brazil
2
Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil
3
Faculty of Biosciences and Aquaculture, Nord University, 8026 Bodø, Norway
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(7), 805; https://doi.org/10.3390/genes11070805
Received: 1 June 2020 / Revised: 5 July 2020 / Accepted: 13 July 2020 / Published: 16 July 2020
(This article belongs to the Special Issue Genetic Research in Aquaculture)
White Spot Syndrome Virus (WSSV) is one of the main threats to farming Litopenaeus vannamei, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) L. vannamei, previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described L. vannamei genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species. View Full-Text
Keywords: innate immunity; RNA-seq; differential expression; WSSV resistance; shrimp innate immunity; RNA-seq; differential expression; WSSV resistance; shrimp
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MDPI and ACS Style

Santos, C.A.; Andrade, S.C.S.; Fernandes, J.M.O.; Freitas, P.D. Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure. Genes 2020, 11, 805. https://doi.org/10.3390/genes11070805

AMA Style

Santos CA, Andrade SCS, Fernandes JMO, Freitas PD. Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure. Genes. 2020; 11(7):805. https://doi.org/10.3390/genes11070805

Chicago/Turabian Style

Santos, Camilla A., Sónia C.S. Andrade, Jorge M.O. Fernandes, and Patrícia D. Freitas 2020. "Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure" Genes 11, no. 7: 805. https://doi.org/10.3390/genes11070805

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