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Article

Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae)

1
Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São, Carlos, São Carlos, SP 13565-905, Brazil
2
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR 84010-330, Brazil
3
Institute of Human Genetics, University Hospital Jena, Jena 07747, Germany
4
Secretaria de Estado de Educação do Mato Grosso—SEDUC-MT, Cuiabá, MT 78049-909, Brazil
*
Author to whom correspondence should be addressed.
Genes 2020, 11(10), 1179; https://doi.org/10.3390/genes11101179
Received: 14 September 2020 / Revised: 6 October 2020 / Accepted: 8 October 2020 / Published: 10 October 2020
(This article belongs to the Special Issue Fish Cytogenetics: Present and Future)
The armored Harttia catfishes present great species diversity and remarkable cytogenetic variation, including different sex chromosome systems. Here we analyzed three new species, H. duriventris, H. villasboas and H. rondoni, using both conventional and molecular cytogenetic techniques (Giemsa-staining and C-banding), including the mapping of repetitive DNAs using fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) experiments. Both H. duriventris and H. villasboas have 2n = ♀56/♂55 chromosomes, and an X1X1X2X2 /X1X2Y sex chromosome system, while a proto or neo-XY system is proposed for H. rondoni (2n = 54♀♂). Single motifs of 5S and 18S rDNA occur in all three species, with the latter being also mapped in the sex chromosomes. The results confirm the general evolutionary trend that has been noticed for the genus: an extensive variation on their chromosome number, single sites of rDNA sequences and the occurrence of multiple sex chromosomes. Comparative genomic analyses with another congeneric species, H. punctata, reveal that the X1X2Y sex chromosomes of these species share the genomic contents, indicating a probable common origin. The remarkable karyotypic variation, including sex chromosomes systems, makes Harttia a suitable model for evolutionary studies focusing on karyotype differentiation and sex chromosome evolution among lower vertebrates. View Full-Text
Keywords: cytogenetics; ribosomal DNA; comparative genomic hybridization; neotropical fishes cytogenetics; ribosomal DNA; comparative genomic hybridization; neotropical fishes
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MDPI and ACS Style

Sassi, F.d.M.C.; Deon, G.A.; Moreira-Filho, O.; Vicari, M.R.; Bertollo, L.A.C.; Liehr, T.; de Oliveira, E.A.; Cioffi, M.B. Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae). Genes 2020, 11, 1179. https://doi.org/10.3390/genes11101179

AMA Style

Sassi FdMC, Deon GA, Moreira-Filho O, Vicari MR, Bertollo LAC, Liehr T, de Oliveira EA, Cioffi MB. Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae). Genes. 2020; 11(10):1179. https://doi.org/10.3390/genes11101179

Chicago/Turabian Style

Sassi, Francisco de M. C., Geize A. Deon, Orlando Moreira-Filho, Marcelo R. Vicari, Luiz A. C. Bertollo, Thomas Liehr, Ezequiel Aguiar de Oliveira, and Marcelo B. Cioffi. 2020. "Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae)" Genes 11, no. 10: 1179. https://doi.org/10.3390/genes11101179

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