Next Article in Journal
Balancing the Photoreceptor Proteome: Proteostasis Network Therapeutics for Inherited Retinal Disease
Next Article in Special Issue
Kinetic Modeling of DUSP Regulation in Herceptin-Resistant HER2-Positive Breast Cancer
Previous Article in Journal
Identification and Expression of SAUR Genes in the CAM Plant Agave
Open AccessArticle

Changes in the Microbial Community Diversity of Oil Exploitation

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China
School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China
Dalian Chivy Biotechnology Limited Company, Liaoning 116023, China
Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Human Genetics Center, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Authors to whom correspondence should be addressed.
The first 3 authors should be regarded as joint First Authors.
Genes 2019, 10(8), 556;
Received: 28 June 2019 / Revised: 15 July 2019 / Accepted: 20 July 2019 / Published: 24 July 2019
PDF [3350 KB, uploaded 24 July 2019]
  |     |  


To systematically evaluate the ecological changes of an active offshore petroleum production system, the variation of microbial communities at several sites (virgin field, wellhead, storage tank) of an oil production facility in east China was investigated by sequencing the V3 to V4 regions of 16S ribosomal ribonucleic acid (rRNA) of microorganisms. In general, a decrease of microbial community richness and diversity in petroleum mining was observed, as measured by operational taxonomic unit (OTU) numbers, α (Chao1 and Shannon indices), and β (principal coordinate analysis) diversity. Microbial community structure was strongly affected by environmental factors at the phylum and genus levels. At the phylum level, virgin field and wellhead were dominated by Proteobacteria, while the storage tank had higher presence of Firmicutes (29.3–66.9%). Specifically, the wellhead displayed a lower presentence of Proteobacteria (48.6–53.4.0%) and a higher presence of Firmicutes (24.4–29.6%) than the virgin field. At the genus level, the predominant genera were Ochrobactrum and Acinetobacter in the virgin field, Lactococcus and Pseudomonas in the wellhead, and Prauseria and Bacillus in the storage tank. Our study revealed that the microbial community structure was strongly affected by the surrounding environmental factors, such as temperature, oxygen content, salinity, and pH, which could be altered because of the oil production. It was observed that the various microbiomes produced surfactants, transforming the biohazard and degrading hydro-carbon. Altering the microbiome growth condition by appropriate human intervention and taking advantage of natural microbial resources can further enhance oil recovery technology. View Full-Text
Keywords: microbial community; 16S rRNA sequencing; microbial enhanced oil recovery technology microbial community; 16S rRNA sequencing; microbial enhanced oil recovery technology

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Liu, J.; Wu, J.; Lin, J.; Zhao, J.; Xu, T.; Yang, Q.; Zhao, J.; Zhao, Z.; Song, X. Changes in the Microbial Community Diversity of Oil Exploitation. Genes 2019, 10, 556.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top