The Genome of the Steller Sea Lion (Eumetopias jubatus)
by
Harwood H. Kwan 1,2
, Luka Culibrk 1,3, Gregory A. Taylor 1, Sreeja Leelakumari 1, Ryan Tan 1, Shaun D. Jackman 1, Kane Tse 1, Tina MacLeod 1, Dean Cheng 1, Eric Chuah 1, Heather Kirk 1, Pawan Pandoh 1, Rebecca Carlsen 1, Yongjun Zhao 1, Andrew J. Mungall 1, Richard Moore 1, Inanc Birol 1,2, Marco A. Marra 1,2, David A.S. Rosen 4,5, Martin Haulena 5 and Steven J. M. Jones 1,2,6,*
Harwood H. Kwan 1,2, Luka Culibrk 1,3, Gregory A. Taylor 1, Sreeja Leelakumari 1, Ryan Tan 1, Shaun D. Jackman 1, Kane Tse 1, Tina MacLeod 1, Dean Cheng 1, Eric Chuah 1, Heather Kirk 1, Pawan Pandoh 1, Rebecca Carlsen 1, Yongjun Zhao 1, Andrew J. Mungall 1, Richard Moore 1, Inanc Birol 1,2, Marco A. Marra 1,2, David A.S. Rosen 4,5, Martin Haulena 5 and Steven J. M. Jones 1,2,6,*
1
Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
2
Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T-1Z4, Canada
3
Department of Graduate Studies, Bioinformatics, University of British Columbia, Vancouver, BC V6T-1Z4, Canada
4
Institute for the Oceans and Fisheries, University of British Columbia, Vancouver, BC V6T-1Z4, Canada
5
Vancouver Aquarium, Vancouver, BC V6G 3E2, Canada
6
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A-1S6, Canada
*
Author to whom correspondence should be addressed.
Genes 2019, 10(7), 486; https://doi.org/10.3390/genes10070486
Received: 15 April 2019 / Revised: 20 June 2019 / Accepted: 21 June 2019 / Published: 26 June 2019
(This article belongs to the Section Animal Genetics and Genomics)
The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
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Keywords:
Steller sea lion; Eumetopias jubatus; genome; microfluidic partitioning; nanopore; marine animal
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Kwan, H.H.; Culibrk, L.; Taylor, G.A.; Leelakumari, S.; Tan, R.; Jackman, S.D.; Tse, K.; MacLeod, T.; Cheng, D.; Chuah, E.; Kirk, H.; Pandoh, P.; Carlsen, R.; Zhao, Y.; Mungall, A.J.; Moore, R.; Birol, I.; Marra, M.A.; Rosen, D.A.; Haulena, M.; Jones, S.J.M. The Genome of the Steller Sea Lion (Eumetopias jubatus). Genes 2019, 10, 486.
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