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Open AccessArticle

Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep

by Yaokun Li 1,2, Lingxuan Kong 1,2, Ming Deng 1,2, Zhiquan Lian 1,2, Yinru Han 1,2, Baoli Sun 1,2, Yongqing Guo 1,2, Guangbin Liu 1,2,* and Dewu Liu 1,2,*
1
College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2
National Local Joint Engineering Research Center of Livestock and Poutry, South China Agricultural University, Guangzhou 510642, China
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(5), 395; https://doi.org/10.3390/genes10050395
Received: 23 March 2019 / Revised: 21 April 2019 / Accepted: 3 May 2019 / Published: 22 May 2019
(This article belongs to the Section Animal Genetics and Genomics)
Heat stress has a severe effect on animal health and can reduce the productivity and reproductive efficiency; it is therefore necessary to explore the molecular mechanism involved in heat stress response, which is helpful for the cultivation of an animal breed with resistance to heat stress. However, little research about heat stress-responsive molecular analysis has been reported in sheep. Therefore, in this study, RNA sequencing (RNA-Seq) was used to investigate the transcriptome profiling in the liver of Hu sheep with and without heat stress. In total, we detected 520 and 22 differentially expressed mRNAs and lncRNAs, respectively. The differentially expressed mRNAs were mainly associated with metabolic processes, the regulation of biosynthetic processes, and the regulation of glucocorticoid; additionally, they were significantly enriched in the heat stress related pathways, including the carbon metabolism, the PPAR signaling pathway, and vitamin digestion and absorption. The co-located differentially expressed lncRNA Lnc_001782 might positively influence the expression of the corresponding genes APOA4 and APOA5, exerting co-regulative effects on the liver function. Thus, we made the hypothesis that Lnc_001782, APOA4 and APOA5 might function synergistically to regulate the anti-heat stress ability in Hu sheep. This study provides a catalog of Hu sheep liver mRNAs and lncRNAs, and will contribute to a better understanding of the molecular mechanism underlying heat stress responses. View Full-Text
Keywords: Hu sheep; heat stress; lncRNA; mRNA; transcriptome analysis Hu sheep; heat stress; lncRNA; mRNA; transcriptome analysis
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Li, Y.; Kong, L.; Deng, M.; Lian, Z.; Han, Y.; Sun, B.; Guo, Y.; Liu, G.; Liu, D. Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep. Genes 2019, 10, 395.

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