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Genes 2019, 10(4), 268;

Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai)

Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung 25457, Korea
The East Coast Research Institute of Life Science, Gangneung-Wonju National University, Gangneung 25457, Korea
TheragenETEX Bio Institute, TheragenETEX Inc., Suwon 16229, Korea
College of Pharmacy, Gachon University, Incheon 21936, Korea
Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 26 December 2018 / Revised: 13 March 2019 / Accepted: 29 March 2019 / Published: 2 April 2019
(This article belongs to the Section Animal Genetics and Genomics)
PDF [3594 KB, uploaded 18 April 2019]


Genetic information of reproduction and growth is essential for sustainable molluscan fisheries and aquaculture management. However, there is limited knowledge regarding the reproductive activity of the commercially important Pacific abalone Haliotis discus hannai. We performed de novo transcriptome sequencing of the ganglia in sexually immature and mature female Pacific abalone to better understand the sexual maturation process and the underlying molecular mechanisms. Of the ~305 million high-quality clean reads, 76,684 transcripts were de novo-assembled with an average length of 741 bp, 28.54% of which were annotated and classified according to Gene Ontology terms. There were 256 differentially expressed genes between the immature and mature abalone. Tandem mass spectrometry analysis, as compared to the predicted-peptide database of abalone ganglia transcriptome unigenes, identified 42 neuropeptide precursors, including 29 validated by peptidomic analyses. Label-free quantification revealed differential occurrences of 18 neuropeptide families between immature and mature abalone, including achatin, FMRFamide, crustacean cardioactive peptide, and pedal peptide A and B that were significantly more frequent at the mature stage. These results represent the first significant contribution to both maturation-related transcriptomic and peptidomic resources of the Pacific abalone ganglia and provide insight into the roles of various neuropeptides in reproductive regulation in marine gastropods. View Full-Text
Keywords: ganglia; gastropod; HCD-MS/MS spectra; maturation-associated neuropeptide; RNA-Seq ganglia; gastropod; HCD-MS/MS spectra; maturation-associated neuropeptide; RNA-Seq

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Kim, M.A.; Markkandan, K.; Han, N.-Y.; Park, J.-M.; Lee, J.S.; Lee, H.; Sohn, Y.C. Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai). Genes 2019, 10, 268.

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