Next Article in Journal
Next-Generation Sequencing Enables Spatiotemporal Resolution of Human Centromere Replication Timing
Previous Article in Journal
The Role and Regulation of Autophagy and the Proteasome During Aging and Senescence in Plants
Article Menu

Export Article

Open AccessArticle
Genes 2019, 10(4), 268; https://doi.org/10.3390/genes10040268

Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai)

1
Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung 25457, Korea
2
The East Coast Research Institute of Life Science, Gangneung-Wonju National University, Gangneung 25457, Korea
3
TheragenETEX Bio Institute, TheragenETEX Inc., Suwon 16229, Korea
4
College of Pharmacy, Gachon University, Incheon 21936, Korea
5
Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 26 December 2018 / Revised: 13 March 2019 / Accepted: 29 March 2019 / Published: 2 April 2019
(This article belongs to the Section Animal Genetics and Genomics)
  |  
PDF [3594 KB, uploaded 18 April 2019]
  |  

Abstract

Genetic information of reproduction and growth is essential for sustainable molluscan fisheries and aquaculture management. However, there is limited knowledge regarding the reproductive activity of the commercially important Pacific abalone Haliotis discus hannai. We performed de novo transcriptome sequencing of the ganglia in sexually immature and mature female Pacific abalone to better understand the sexual maturation process and the underlying molecular mechanisms. Of the ~305 million high-quality clean reads, 76,684 transcripts were de novo-assembled with an average length of 741 bp, 28.54% of which were annotated and classified according to Gene Ontology terms. There were 256 differentially expressed genes between the immature and mature abalone. Tandem mass spectrometry analysis, as compared to the predicted-peptide database of abalone ganglia transcriptome unigenes, identified 42 neuropeptide precursors, including 29 validated by peptidomic analyses. Label-free quantification revealed differential occurrences of 18 neuropeptide families between immature and mature abalone, including achatin, FMRFamide, crustacean cardioactive peptide, and pedal peptide A and B that were significantly more frequent at the mature stage. These results represent the first significant contribution to both maturation-related transcriptomic and peptidomic resources of the Pacific abalone ganglia and provide insight into the roles of various neuropeptides in reproductive regulation in marine gastropods. View Full-Text
Keywords: ganglia; gastropod; HCD-MS/MS spectra; maturation-associated neuropeptide; RNA-Seq ganglia; gastropod; HCD-MS/MS spectra; maturation-associated neuropeptide; RNA-Seq
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kim, M.A.; Markkandan, K.; Han, N.-Y.; Park, J.-M.; Lee, J.S.; Lee, H.; Sohn, Y.C. Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai). Genes 2019, 10, 268.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top