miR319 was the first plant miRNA discovered via forward genetic mutation screening. In this study, we found that miR319 family members had similar sequences but different expression patterns in Brassica campestris
and Arabidopsis thaliana.
RT-PCR analysis revealed that Bra-MIR319a
had similar expression patterns and were widely expressed in plant development, whereas Bra-MIR319b
could only be detected in stems. The overexpression of each Bra-MIR319
family member in Arabidopsis
could inhibit cell division and function in leaf and petal morphogenesis. Bra-miR319a formed a new regulatory relationship after whole genome triplication, and Bra-MIR319a
overexpressing in Arabidopsis
led to the degradation of pollen content and affected the formation of intine, thereby causing pollen abortion. Our results suggest that Bra-MIR319
family members have functional similarity and difference in plant development.
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