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Open AccessArticle

Functional Similarity and Difference among Bra-MIR319 Family in Plant Development

by Ziwei Hu 1,2, Tingting Liu 1,2 and Jiashu Cao 1,2,3,*
1
Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
2
Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China
3
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(12), 952; https://doi.org/10.3390/genes10120952
Received: 29 October 2019 / Revised: 10 November 2019 / Accepted: 13 November 2019 / Published: 21 November 2019
miR319 was the first plant miRNA discovered via forward genetic mutation screening. In this study, we found that miR319 family members had similar sequences but different expression patterns in Brassica campestris and Arabidopsis thaliana. RT-PCR analysis revealed that Bra-MIR319a and Bra-MIR319c had similar expression patterns and were widely expressed in plant development, whereas Bra-MIR319b could only be detected in stems. The overexpression of each Bra-MIR319 family member in Arabidopsis could inhibit cell division and function in leaf and petal morphogenesis. Bra-miR319a formed a new regulatory relationship after whole genome triplication, and Bra-MIR319a overexpressing in Arabidopsis led to the degradation of pollen content and affected the formation of intine, thereby causing pollen abortion. Our results suggest that Bra-MIR319 family members have functional similarity and difference in plant development. View Full-Text
Keywords: Brassica campestris; Bra-miR319 family; leaf and petal morphogenesis; pollen development Brassica campestris; Bra-miR319 family; leaf and petal morphogenesis; pollen development
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Hu, Z.; Liu, T.; Cao, J. Functional Similarity and Difference among Bra-MIR319 Family in Plant Development. Genes 2019, 10, 952.

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