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m6A-Dependent RNA Dynamics in T Cell Differentiation

Center for Genomic Science, Fondazione Istituto Italiano di Tecnologia, 20139 Milan, Italy
Physics Department and INFN, University of Turin, 10125 Turin, Italy
Author to whom correspondence should be addressed.
Genes 2019, 10(1), 28;
Received: 22 November 2018 / Revised: 15 December 2018 / Accepted: 27 December 2018 / Published: 8 January 2019
(This article belongs to the Special Issue RNA Modifications)
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N6-methyladenosine (m6A) is the most abundant RNA modification. It has been involved in the regulation of RNA metabolism, including degradation and translation, in both physiological and disease conditions. A recent study showed that m6A-mediated degradation of key transcripts also plays a role in the control of T cells homeostasis and IL-7 induced differentiation. We re-analyzed the omics data from that study and, through the integrative analysis of total and nascent RNA-seq data, we were able to comprehensively quantify T cells RNA dynamics and how these are affected by m6A depletion. In addition to the expected impact on RNA degradation, we revealed a broader effect of m6A on RNA dynamics, which included the alteration of RNA synthesis and processing. Altogether, the combined action of m6A on all major steps of the RNA life-cycle closely re-capitulated the observed changes in the abundance of premature and mature RNA species. Ultimately, our re-analysis extended the findings of the initial study, focused on RNA stability, and proposed a yet unappreciated role for m6A in RNA synthesis and processing dynamics. View Full-Text
Keywords: RNA-seq; RNA dynamics; m6A; RNA modifications; RNA metabolic labeling; mathematical modeling RNA-seq; RNA dynamics; m6A; RNA modifications; RNA metabolic labeling; mathematical modeling

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Furlan, M.; Galeota, E.; De Pretis, S.; Caselle, M.; Pelizzola, M. m6A-Dependent RNA Dynamics in T Cell Differentiation. Genes 2019, 10, 28.

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