Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Sclerocorneal Tissue
2.2. Isolation and Cultivation of Limbal Epithelial Cells
2.3. Notch Signaling Activation in the Limbal Epithelial Cells
2.4. Mitotic Arrest of the Stratified Limbal Epithelial Cultures and Analysis of Divisions
2.5. Immunohistochemistry
2.6. Quantitative RT-PCR (qRT-PCR)
2.7. Air-lifting Induction
2.8. Quantification and Statistical Analysis
3. Results
3.1. Jag1 is Expressed in the Limbal Epithelium and Activates Notch Signaling in LECs
3.2. Jag1 Promoted Differentiation of LECs In Vitro
3.3. Jag1-Mediated Notch Activation Reduced Stratification and Promoted Differentiation of LECs upon Air-Lifting
3.4. Jag1 Decreased Asymmetric Divisions in Basal LECs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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González, S.; Halabi, M.; Ju, D.; Tsai, M.; Deng, S.X. Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium. Cells 2020, 9, 1945. https://doi.org/10.3390/cells9091945
González S, Halabi M, Ju D, Tsai M, Deng SX. Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium. Cells. 2020; 9(9):1945. https://doi.org/10.3390/cells9091945
Chicago/Turabian StyleGonzález, Sheyla, Maximilian Halabi, David Ju, Matthew Tsai, and Sophie X. Deng. 2020. "Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium" Cells 9, no. 9: 1945. https://doi.org/10.3390/cells9091945