Cellular Model of Endotoxin Tolerance in Astrocytes: Role of Interleukin 10 and Oxylipins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Primary Cell Culture
2.3. Measurement of the Relative RNA Expression Level
2.4. Western Blot Analysis
2.5. UPLC-MS/MS Conditions and Sample Preparation
2.6. Determination of TNFα and IL-10 by Enzyme-Linked Immunoassay
2.7. Experimental Data Analysis and Statistics
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are commercially available. |
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Chistyakov, D.V.; Astakhova, A.A.; Azbukina, N.V.; Goriainov, S.V.; Chistyakov, V.V.; Sergeeva, M.G. Cellular Model of Endotoxin Tolerance in Astrocytes: Role of Interleukin 10 and Oxylipins. Cells 2019, 8, 1553. https://doi.org/10.3390/cells8121553
Chistyakov DV, Astakhova AA, Azbukina NV, Goriainov SV, Chistyakov VV, Sergeeva MG. Cellular Model of Endotoxin Tolerance in Astrocytes: Role of Interleukin 10 and Oxylipins. Cells. 2019; 8(12):1553. https://doi.org/10.3390/cells8121553
Chicago/Turabian StyleChistyakov, Dmitry V., Alina A. Astakhova, Nadezda V. Azbukina, Sergei V. Goriainov, Viktor V. Chistyakov, and Marina G. Sergeeva. 2019. "Cellular Model of Endotoxin Tolerance in Astrocytes: Role of Interleukin 10 and Oxylipins" Cells 8, no. 12: 1553. https://doi.org/10.3390/cells8121553