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Cells 2018, 7(7), 65; https://doi.org/10.3390/cells7070065

Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites

Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark
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Received: 25 May 2018 / Revised: 19 June 2018 / Accepted: 20 June 2018 / Published: 22 June 2018
(This article belongs to the Special Issue Comparative Biology of Centrosomal Structures in Eukaryotes)
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Abstract

Centriolar satellites (CS) are small proteinaceous granules that cluster around the centrosome and serve as cargo vehicles for centrosomal proteins. It is generally accepted that CS support a number of canonical and specialized centrosome functions. Consequently, these highly dynamic structures are the target of regulation by several cellular signalling pathways. Two decades of research have led to the identification of a large number of molecular components and new biological roles of CS. Here, we summarize the latest advances in the continuous efforts to uncover the compositional, functional, dynamic and regulatory aspects of CS. We also report on our discovery that osmotic stress conditions render CS immobile and insensitive to remodelling. Upon a range of p38-activating stimuli, MK2 phosphorylates the CS component CEP131, resulting in 14-3-3 binding and a block to CS formation. This normally manifests as a rapid cellular depletion of satellites. In the case of osmotic stress, a potent inducer of p38 activity, CS translocation and dissolution is blocked, with the net result that satellites persist in an immobile state directly adjacent to the centrosome. Our results highlight a unique scenario where p38 activation and CS depletion is uncoupled, with potential implications for physiological and pathological osmotic stress responses. View Full-Text
Keywords: centriolar satellites; cell stress; osmotic stress; MAP kinase signalling; p38; MK2 centriolar satellites; cell stress; osmotic stress; MAP kinase signalling; p38; MK2
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Nielsen, J.C.; Nordgaard, C.; Tollenaere, M.A.X.; Bekker-Jensen, S. Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites. Cells 2018, 7, 65.

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