Next Article in Journal
Unconventional Secretion and Intercellular Transfer of Mutant Huntingtin
Previous Article in Journal
A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Cells 2018, 7(6), 58;

Effect of Notch and PARP Pathways’ Inhibition in Leukemic Cells

Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102A, 10000 Zagreb, Croatia
Division of Molecular Biology, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
Author to whom correspondence should be addressed.
Received: 15 May 2018 / Revised: 11 June 2018 / Accepted: 12 June 2018 / Published: 14 June 2018
Full-Text   |   PDF [1898 KB, uploaded 14 June 2018]   |  


Differentiation of blood cells is one of the most complex processes in the body. It is regulated by the action of transcription factors in time and space which creates a specific signaling network. In the hematopoietic signaling system, Notch is one of the main regulators of lymphocyte development. The aim of this study was to get insight into the regulation of Notch signalization and the influence of poly(ADP-ribose)polymerase (PARP) activity on this process in three leukemia cell lines obtained from B and T cells. PARP1 is an enzyme involved in posttranslational protein modification and chromatin structure changes. B and T leukemia cells were treated with Notch and PARP inhibitors, alone or in combination, for a prolonged period. The cells did not show cell proliferation arrest or apoptosis. Analysis of gene and protein expression set involved in Notch and PARP pathways revealed increase in JAGGED1 expression after PARP1 inhibition in B cell lines and changes in Ikaros family members in both B and T cell lines after γ-secretase inhibition. These data indicate that Notch and PARP inhibition, although not inducing differentiation in leukemia cells, induce changes in signaling circuits and chromatin modelling factors. View Full-Text
Keywords: B and T leukemia cell line; Notch; PARP1; Ikaros; Jagged; gene expression B and T leukemia cell line; Notch; PARP1; Ikaros; Jagged; gene expression

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Horvat, L.; Antica, M.; Matulić, M. Effect of Notch and PARP Pathways’ Inhibition in Leukemic Cells. Cells 2018, 7, 58.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top