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Open AccessFeature PaperCommunication

Identification of Novel Hemangioblast Genes in the Early Chick Embryo

1
CEDOC, Chronic Diseases Research Centre, NOVA Medical School, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisbon, Portugal
2
Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156 Oeiras, Portugal
*
Author to whom correspondence should be addressed.
Received: 10 December 2017 / Revised: 17 January 2018 / Accepted: 27 January 2018 / Published: 31 January 2018
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Abstract

During early vertebrate embryogenesis, both hematopoietic and endothelial lineages derive from a common progenitor known as the hemangioblast. Hemangioblasts derive from mesodermal cells that migrate from the posterior primitive streak into the extraembryonic yolk sac. In addition to primitive hematopoietic cells, recent evidence revealed that yolk sac hemangioblasts also give rise to tissue-resident macrophages and to definitive hematopoietic stem/progenitor cells. In our previous work, we used a novel hemangioblast-specific reporter to isolate the population of chick yolk sac hemangioblasts and characterize its gene expression profile using microarrays. Here we report the microarray profile analysis and the identification of upregulated genes not yet described in hemangioblasts. These include the solute carrier transporters SLC15A1 and SCL32A1, the cytoskeletal protein RhoGap6, the serine protease CTSG, the transmembrane receptor MRC1, the transcription factors LHX8, CITED4 and PITX1, and the previously uncharacterized gene DIA1R. Expression analysis by in situ hybridization showed that chick DIA1R is expressed not only in yolk sac hemangioblasts but also in particular intraembryonic populations of hemogenic endothelial cells, suggesting a potential role in the hemangioblast-derived hemogenic lineage. Future research into the function of these newly identified genes may reveal novel important regulators of hemangioblast development. View Full-Text
Keywords: chicken embryo; yolk sac; hemangioblast; microarray analysis; novel genes chicken embryo; yolk sac; hemangioblast; microarray analysis; novel genes
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Serrado Marques, J.; Teixeira, V.; Jacinto, A.; Tavares, A.T. Identification of Novel Hemangioblast Genes in the Early Chick Embryo. Cells 2018, 7, 9.

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