Identification of Novel Hemangioblast Genes in the Early Chick Embryo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Embryo Ex Ovo Electroporation
2.2. Immunohistochemistry
2.3. Microarray Data Analysis
2.4. In Situ Hybridization
3. Results and Discussion
3.1. Gene Expression Analysis of the Hemangioblast Transcriptome
3.2. Identification of Novel Hemangioblast Genes
3.3. Expression Pattern of DIA1R in the Chick Embryo
Acknowledgments
Author Contributions
Conflicts of Interest
References
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FC b | Gene Symbol | Gene Name | Gene ID | Molecular Function | Biological Function | Expression in Early Embryos |
---|---|---|---|---|---|---|
+5.1 | SLC15A1 | Solute carrier family 15, member 1 | 378789 | Membrane transporter | Oligopeptide transport | - |
+4.2 | C1HXorf36 (DIA1R) | Chromosome 1 open reading frame, human CXorf36 (Deleted in Autism 1 Related) | 418555 | - | - | (this study) |
+4.19 | SLC32A1 | Solute carrier family 32, member 1 | 419167 | Vesicular transporter | GABA vesicular transporter | - |
+4.15 | SOX7 | SRY (sex determining region Y)-box 7 | 771337 | Transcription factor | Vasculogenesis and hematopoiesis | Angioblasts |
+3.73 | LMO2 | LIM domain only 2 | 374129 | Transcription factor | Hematopoiesis | Hematopoietic progenitors |
+3.7 | CTSG | Cathepsin G | 426049 | Serine protease | Tissue remodeling | Myeloid progenitors |
+3.62 | TAL1 (SCL) | T-cell acute lymphocytic leukemia 1 (stem cell leukemia) | 396298 | Transcription factor | Hematopoiesis | Hematopoietic progenitors |
+3.51 | RUNX1 | Runt-related transcription factor 1 | 396152 | Transcription factor | Hematopoiesis | Blood islands |
+3.44 | EGR1 | Early growth response 1 | 373931 | Transcription factor | HSPC proliferation | Vasculogenic mesoderm |
+3.2 | MRC1 | Mannose receptor C-type 1 | 420516 | Membrane receptor | Endocytosis | Blood islands |
+3.17 | KLHL6 | Kelch-like 6 | 424762 | Transcription factor | Lymphocyte differentiation | - |
+3.3 | SPI1 (PU.1) | Spleen focus forming virus (SFFV) proviral integration oncogene spi1 | 395879 | Transcription factor | Hematopoiesis | Hematopoietic progenitors |
+3.07 | RhoGap6 | Similar to Rho-GTPase-activating protein 6 (LOC422284 locus) | 422284 | Cytoskeleton regulator | Actin remodeling | - |
+2.77 | LHX8 | LIM homeobox 8 | 424721 | Transcription factor | Neurogenesis | Blood islands |
+2.71 | FLT1 (VEGFR1) | Fms-related tyrosine kinase 1 (vascular endothelial growth factor receptor 1) | 374100 | Receptor tyrosine kinase | Vasculogenesis/Angiogenesis | Hemangioblasts and endothelial cells |
+2.63 | SOX18 | SRY (sex determining region Y)-box 18 | 374200 | Transcription factor | Vasculogenesis | Blood islands |
+2.49 | CDH5 | Cadherin 5, type 2, VE-cadherin (vascular epithelium) | 374068 | Cell adhesion molecule | Vasculogenesis/Angiogenesis | Endothelial cells |
+2.39 | CD34 | Hematopoietic progenitor cell antigen CD34 | 419856 | Cell surface antigen | - | Hematopoietic progenitors |
+2.29 | FLT4 (VEGFR3) | Fms-related tyrosine kinase 4 (vascular endothelial growth factor receptor 3) | 395742 | Receptor tyrosine kinase | Angiogenesis | Blood islands and endothelial cells |
+2.23 | CITED4 | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 | 395465 | Transcription regulator | in vitro cardiogenesis | Blood islands |
+2.2 | PITX1 | Paired-like homeodomain 1 | 374201 | Transcription factor | Pituitary and hindlimb development | Posterior extraembryonic mesoderm |
+2.09 | Fli1 | Friend leukemia virus integration 1 gene | 419723 | Transcription factor | Vasculogenesis and hematopoiesis | Endothelial and erythroid progenitors |
+1.96 | HHEX | Hematopoietically expressed homeobox | 396182 | Transcription factor | Vasculogenesis and hematopoiesis | Blood islands |
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Serrado Marques, J.; Teixeira, V.; Jacinto, A.; Tavares, A.T. Identification of Novel Hemangioblast Genes in the Early Chick Embryo. Cells 2018, 7, 9. https://doi.org/10.3390/cells7020009
Serrado Marques J, Teixeira V, Jacinto A, Tavares AT. Identification of Novel Hemangioblast Genes in the Early Chick Embryo. Cells. 2018; 7(2):9. https://doi.org/10.3390/cells7020009
Chicago/Turabian StyleSerrado Marques, José, Vera Teixeira, António Jacinto, and Ana Teresa Tavares. 2018. "Identification of Novel Hemangioblast Genes in the Early Chick Embryo" Cells 7, no. 2: 9. https://doi.org/10.3390/cells7020009