Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater
Abstract
:1. Introduction
2. Materials and Methods
2.1. Surgical Procedure and SAH Induction
2.2. Retrograde Labeling of Trigeminal Afferents
2.3. Experimental Groups
2.4. Neurological Examination
2.5. Immunohistochemistry
2.6. Immunohistochemical Imaging and Image Analysis
2.6.1. Image Acquisition and Line Scan Analysis in Dura Mater Preparation
2.6.2. Immunohistochemical Analysis in the TG
2.6.3. Immunohistochemical Analysis in the TNC
2.7. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.8. Measuring CGRP Release from Meningeal Afferents
2.9. Statistics
3. Results
3.1. Neurological Evaluation of the Single-Injection SAH Model
3.2. Macroscopic Inspection of the Brain after SAH
3.3. SAH Depletes CGRP in Perivascular Nerves in the Rat Dura Mater
3.4. SAH Reduces CGRP Release from the Rat Dura Mater
3.5. SAH Reduces the Proportion of Intensively CGRP-IR Neuronal Cell Bodies in the TG
3.6. SAH Attenuates CGRP mRNA Expression in the TG
3.7. SAH Depletes CGRP also in the Central Trigeminal Projections in the Trigeminal Nucleus Caudalis (TNC)
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CGRP | calcitonin gene-related peptide |
CSF | cerebrospinal fluid |
CV | cerebral vasospasm |
ELISA | Enzyme-linked Immunosorbent Assay |
ICP | intracranial pressure |
IR | immuno-reactive |
qRT-PCR | Quantitative Real-Time Polymerase Chain Reaction |
SEM | standard error of the mean |
SIF | synthetic interstitial fluid |
SAH | subarachnoid hemorrhage |
TNC | trigeminal nucleus caudalis |
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Experimental Group | CTL | SHAM | aCSF | SAH | FG |
---|---|---|---|---|---|
surgical procedure | - | + | + | + | + |
intracisternal injection | - | - | 200 μL aCSF | 200 μL blood | 15 μL FG in aCSF |
survival time | - | 72 h | 72 h | 72 h | 5 days |
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Masood, T.; Lakatos, S.; Kis, G.; Ignácz, M.; Domoki, F.; Rosta, J. Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater. Cells 2024, 13, 653. https://doi.org/10.3390/cells13080653
Masood T, Lakatos S, Kis G, Ignácz M, Domoki F, Rosta J. Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater. Cells. 2024; 13(8):653. https://doi.org/10.3390/cells13080653
Chicago/Turabian StyleMasood, Thannoon, Szandra Lakatos, Gyöngyi Kis, Melissza Ignácz, Ferenc Domoki, and Judit Rosta. 2024. "Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater" Cells 13, no. 8: 653. https://doi.org/10.3390/cells13080653