Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Virus Stocks
2.2. RNA Sequencing and Data Analysis
2.3. Viral RNA Extraction and qRT-PCR Experiments
2.4. Gene Expression Assay by Real-Time qPCR
2.5. Dual-Luciferase Reporter Assay
2.6. Western Blot Assay
2.7. Statistical Analysis
3. Results
3.1. Comparative Analysis of the Gene Expression Profiles after SARS-CoV-2, panH1N1, and H7N9 Infection
3.2. Functional Categorization and Pathway Analysis of the Common Differentially Expressed Genes (DEGs) among the Three Viral Infections
3.3. Functional Categorization and Pathway Analysis of Specific DEGs in SARS-CoV-2 Infection
3.4. LncRNA-34087.27 Upregulates IRF1 Expression by Acting as a Competitive Endogenous RNA in SARS-CoV-2, panH1N1, and H7N9 Infections
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, M.; Li, J.; Deng, S.; Fan, H.; Peng, Y.; Ye, G.; Wang, J.; Wei, J.; Jiang, X.; Xu, Z.; et al. Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells. Cells 2022, 11, 487. https://doi.org/10.3390/cells11030487
Yang M, Li J, Deng S, Fan H, Peng Y, Ye G, Wang J, Wei J, Jiang X, Xu Z, et al. Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells. Cells. 2022; 11(3):487. https://doi.org/10.3390/cells11030487
Chicago/Turabian StyleYang, Minghui, Jin Li, Shoulong Deng, Hao Fan, Yun Peng, Guoguo Ye, Jun Wang, Jinli Wei, Xiao Jiang, Zhixiang Xu, and et al. 2022. "Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells" Cells 11, no. 3: 487. https://doi.org/10.3390/cells11030487
APA StyleYang, M., Li, J., Deng, S., Fan, H., Peng, Y., Ye, G., Wang, J., Wei, J., Jiang, X., Xu, Z., Qing, L., Wang, F., Yang, Y., & Liu, Y. (2022). Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells. Cells, 11(3), 487. https://doi.org/10.3390/cells11030487