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Article

Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures

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Department of Biotechnology, Institute of Cell and Tissue Culture Technologies, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
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Health—Institute for Biomedicine and Health Sciences, JOANNEUM RESEARCH Forschungsgesellschaft mbH, Neue Stiftingtalstrasse 2, 8010 Graz, Austria
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Faculty of Chemistry and Pharmacy, Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany
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Department of Gynecology and Obstetrics Tulln, Karl Landsteiner University of Health Sciences, Alter Ziegelweg 10, 3430 Tulln, Austria
*
Author to whom correspondence should be addressed.
Academic Editors: Friedrich Jung, Michael Raghunath and Anna Blocki
Cells 2022, 11(3), 412; https://doi.org/10.3390/cells11030412
Received: 15 December 2021 / Revised: 21 January 2022 / Accepted: 22 January 2022 / Published: 25 January 2022
(This article belongs to the Collection Advances in Cell Culture and Tissue Engineering)
The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation. View Full-Text
Keywords: 3D culture; perfusion bioreactor; monitoring platform; sampling; analytics; cells; microenvironment; open flow microperfusion; sensors; 3R 3D culture; perfusion bioreactor; monitoring platform; sampling; analytics; cells; microenvironment; open flow microperfusion; sensors; 3R
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MDPI and ACS Style

Kreß, S.; Schaller-Ammann, R.; Feiel, J.; Wegener, J.; Priedl, J.; Dietrich, W.; Kasper, C.; Egger, D. Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures. Cells 2022, 11, 412. https://doi.org/10.3390/cells11030412

AMA Style

Kreß S, Schaller-Ammann R, Feiel J, Wegener J, Priedl J, Dietrich W, Kasper C, Egger D. Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures. Cells. 2022; 11(3):412. https://doi.org/10.3390/cells11030412

Chicago/Turabian Style

Kreß, Sebastian, Roland Schaller-Ammann, Jürgen Feiel, Joachim Wegener, Joachim Priedl, Wolf Dietrich, Cornelia Kasper, and Dominik Egger. 2022. "Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures" Cells 11, no. 3: 412. https://doi.org/10.3390/cells11030412

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