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Article

Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro

by
Atsushi Fuku
1,
Yasuhiko Taki
1,
Yuka Nakamura
2,
Hironori Kitajima
1,
Takashi Takaki
3,
Terutsugu Koya
4,5,
Ikuhiro Tanida
6,
Kaori Nozaki
7,
Hiroshi Sunami
8,
Hiroaki Hirata
1,
Yoshiyuki Tachi
1,
Togen Masauji
7,
Naoki Yamamoto
9,10,
Yasuhito Ishigaki
2,4,
Shigetaka Shimodaira
4,5,
Yusuke Shimizu
11,
Toru Ichiseki
1,*,
Ayumi Kaneuji
1,
Satoshi Osawa
6 and
Norio Kawahara
1
1
Department of Orthopedic Surgery, Kanazawa Medical University, Kahoku 920-0293, Ishikawa, Japan
2
Medical Research Institute, Kanazawa Medical University, Kahoku 920-0293, Ishikawa, Japan
3
Division of Electron Microscopy, Showa University, Tokyo 142-8555, Japan
4
Center for Regenerative Medicine, Kanazawa Medical University Hospital, Kahoku 920-0293, Ishikawa, Japan
5
Department of Regenerative Medicine, Kanazawa Medical University, Kahoku 920-0293, Ishikawa, Japan
6
Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan 924-0838, Ishikawa, Japan
7
Department of Pharmacy, Kanazawa Medical University Hospital, Kahoku 920-0293, Ishikawa, Japan
8
Advanced Medical Research Center, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nakagami 903-0215, Okinawa, Japan
9
Center for Clinical Trial and Research Support, Research Promotion and Support Headquarters, Fujita Health University, Toyoake 470-1192, Aichi, Japan
10
Department of Ophthalmology, Kanazawa Medical University, Kahoku 920-0293, Ishikawa, Japan
11
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, University of the Ryukyus, Nakagami 903-0215, Okinawa, Japan
 
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Author to whom correspondence should be addressed.
Cells 2022, 11(3), 337; https://doi.org/10.3390/cells11030337
Submission received: 16 December 2021 / Revised: 12 January 2022 / Accepted: 13 January 2022 / Published: 20 January 2022
(This article belongs to the Section Cell Methods)
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Abstract

Osteoarthritis (OA) is an irreversible degenerative condition causing bone deformation in the joints and articular cartilage degeneration with chronic pain and impaired movement. Adipose-derived stem cell (ADSC) or crushed adipose tissue injection into the joint cavity reportedly improve knee function and symptoms, including pain. Stem cell spheroids may be promising treatment options due to their anti-inflammatory and enhanced tissue regeneration/repair effects. Herein, to form human ADSC spheroids, we used first SphereRing® (Fukoku Co., Ltd., Ageo, Japan), a newly developed rotating donut-shaped tube and determined their characteristics by DNA microarray of mRNA analysis. The variable gene expression cluster was then identified and validated by RT-PCR. Gene expression fluctuations were observed, such as COL15A1 and ANGPTL2, related to vascular endothelial cells and angiogenesis, and TNC, involved in tissue formation. In addition, multiplex cytokine analysis in the medium revealed significant cytokines and growth factors production increase of IL-6, IL-10, etc. However, ADSC administration into the joint cavity involves their contact with the synovial fluid (SF). Therefore, we examined how SF collected from OA patient joint cavities affect 2D-culture ADSCs and ADSC spheroids and observed SF induced cell death. ADSC spheroids could become promising OA treatment options, although studying the administration methods and consider their interaction with SF is essential.
Keywords: spheroid; adipose-derived stem cell; synovial fluid; knee osteoarthritis; cell methods spheroid; adipose-derived stem cell; synovial fluid; knee osteoarthritis; cell methods

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MDPI and ACS Style

Fuku, A.; Taki, Y.; Nakamura, Y.; Kitajima, H.; Takaki, T.; Koya, T.; Tanida, I.; Nozaki, K.; Sunami, H.; Hirata, H.; et al. Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro. Cells 2022, 11, 337. https://doi.org/10.3390/cells11030337

AMA Style

Fuku A, Taki Y, Nakamura Y, Kitajima H, Takaki T, Koya T, Tanida I, Nozaki K, Sunami H, Hirata H, et al. Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro. Cells. 2022; 11(3):337. https://doi.org/10.3390/cells11030337

Chicago/Turabian Style

Fuku, Atsushi, Yasuhiko Taki, Yuka Nakamura, Hironori Kitajima, Takashi Takaki, Terutsugu Koya, Ikuhiro Tanida, Kaori Nozaki, Hiroshi Sunami, Hiroaki Hirata, and et al. 2022. "Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro" Cells 11, no. 3: 337. https://doi.org/10.3390/cells11030337

APA Style

Fuku, A., Taki, Y., Nakamura, Y., Kitajima, H., Takaki, T., Koya, T., Tanida, I., Nozaki, K., Sunami, H., Hirata, H., Tachi, Y., Masauji, T., Yamamoto, N., Ishigaki, Y., Shimodaira, S., Shimizu, Y., Ichiseki, T., Kaneuji, A., Osawa, S., & Kawahara, N. (2022). Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro. Cells, 11(3), 337. https://doi.org/10.3390/cells11030337

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