Pharmacological Inhibition of Spleen Tyrosine Kinase Suppressed Neuroinflammation and Cognitive Dysfunction in LPS-Induced Neurodegeneration Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal
- Control group (Saline for 9 days).
- LPS group (0.25 mg/kg for 9 days).
- LPS + BAY61-3606 (0.25 mg/kg + 10 mg/kg for 9 days).
- BAY61-3606 (10 mg/kg for 9 days).
2.2. Y-Maze and Passive Avoidance Task
2.3. Tissue Processing
2.4. Nissl Staining
2.5. Immunofluorescence
2.6. Cell Culture
2.7. Transwell Co-Culture Assay
2.8. Western Blot Analysis
2.9. Antibodies
2.10. RNA Isolation and Quantitative PCR
2.11. Statistical Analysis
3. Results
3.1. Pharmacological Inhibition of Syk Mitigated the Expression of Microglial Cells and Inflammatory Mediators in LPS-Induced Mouse
3.2. Pharmacological Inhibition of Syk Mitigated the Inflammatory Mediators in LPS-Induced Mouse
3.3. Pharmacological Inhibition of Syk Mitigated LPS-Induced Inflammation in BV2 Cells
3.4. Pharmacological Inhibition of Syk Mitigated the Apoptotic Cell Death in LPS-Induced Mice
3.5. Pharmacological Inhibition of Syk Protected against Neurotoxin-Mediated Neuronal Cell Death
3.6. Pharmacological Inhibition of Syk Restored Synaptic Dysfunctions, Cognitive, and Spatial Working Memory Impairment in LPS-Injected Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, M.W.; Choe, K.; Park, J.S.; Lee, H.J.; Kang, M.H.; Ahmad, R.; Kim, M.O. Pharmacological Inhibition of Spleen Tyrosine Kinase Suppressed Neuroinflammation and Cognitive Dysfunction in LPS-Induced Neurodegeneration Model. Cells 2022, 11, 1777. https://doi.org/10.3390/cells11111777
Kim MW, Choe K, Park JS, Lee HJ, Kang MH, Ahmad R, Kim MO. Pharmacological Inhibition of Spleen Tyrosine Kinase Suppressed Neuroinflammation and Cognitive Dysfunction in LPS-Induced Neurodegeneration Model. Cells. 2022; 11(11):1777. https://doi.org/10.3390/cells11111777
Chicago/Turabian StyleKim, Min Woo, Kyonghwan Choe, Jun Sung Park, Hyeon Jin Lee, Min Hwa Kang, Riaz Ahmad, and Myeong Ok Kim. 2022. "Pharmacological Inhibition of Spleen Tyrosine Kinase Suppressed Neuroinflammation and Cognitive Dysfunction in LPS-Induced Neurodegeneration Model" Cells 11, no. 11: 1777. https://doi.org/10.3390/cells11111777