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Establishing F1A-CreERT2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes

by 1,2,†, 3,†, 3,†, 4, 4 and 3,5,6,*
Institute of Biomedical Sciences, Mackay Medical College, New Taipei City 252, Taiwan
Department of Audiology and Speech Language Pathology, Mackay Medical College, New Taipei City 252, Taiwan
Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 350, Taiwan
Department of Research and Development, National Laboratory Animal Center, National Applied Research Laboratories, Tainan 700, Taiwan
Department of Life Sciences, National Chung Hsing University, Taichung 400, Taiwan
Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: T.K.S. Kumar
Cells 2022, 11(1), 121;
Received: 13 November 2021 / Revised: 24 December 2021 / Accepted: 25 December 2021 / Published: 30 December 2021
(This article belongs to the Collection Fibroblast Growth Factors: Pathophysiology and Therapeutics)
Fibroblast growth factor 1 (FGF1) regulates many biological and physiological processes. In mice, Fgf1 gene contains at least three upstream promoters and are alternatively spliced to the first protein coding exon, giving rise to different Fgf1 mRNA variants (1A, 1B and 1G). Among them, the Fgf1A transcript is predominantly expressed in the heart. FGF1 can induce cardiomyocyte regeneration and cardiogenesis in vitro and in vivo. Here, we generated a novel mouse line using the Fgf1A promoter (F1A) driving the expression of the inducible Cre recombinase (CreERT2). We firstly demonstrated that the highest mRNA expression of CreERT2 were detected in the heart specifically of F1A-CreERT2 mice, similar to that of Fgf1A mRNA. The F1A-CreERT2 mice were crossed with ROSA26 mice, and the F1 mice were analyzed. The LacZ-positive signals were detected exclusively in the heart after tamoxifen administration. The CreERT2-mediated recombination in the tissues is monitored through LacZ-positive signals, indicating the in situ localization of F1A-positive cells. Consistently, these F1A-positive cells with RFP-positive signals or LacZ-positive blue signals were co-localized with cardiomyocytes expressing cardiac troponin T, suggesting cardiomyocyte-specific activation of Fgf1A promoter. Our data suggested that the F1A-CreERT2 mouse line could be used for time-dependent and lineage tracing of Fgf1A-expressing cells in vivo. View Full-Text
Keywords: FGF1; Fgf1A promoter; inducible Cre; cardiomyocyte FGF1; Fgf1A promoter; inducible Cre; cardiomyocyte
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MDPI and ACS Style

Hsu, Y.-C.; Chung, Y.-F.; Chen, M.-S.; Wang, C.-K.; Jiang, S.-T.; Chiu, I.-M. Establishing F1A-CreERT2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes. Cells 2022, 11, 121.

AMA Style

Hsu Y-C, Chung Y-F, Chen M-S, Wang C-K, Jiang S-T, Chiu I-M. Establishing F1A-CreERT2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes. Cells. 2022; 11(1):121.

Chicago/Turabian Style

Hsu, Yi-Chao, Yu-Fen Chung, Mei-Shu Chen, Chi-Kuang Wang, Si-Tse Jiang, and Ing-Ming Chiu. 2022. "Establishing F1A-CreERT2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes" Cells 11, no. 1: 121.

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