Next Article in Journal
Upscaling Evapotranspiration with Parsimonious Models in a North Carolina Vineyard
Next Article in Special Issue
Analysis of Distribution of Selected Bioactive Compounds in Camelina sativa from Seeds to Pomace and Oil
Previous Article in Journal
Impact of Nitrogen Fertilization on Tuber Yield, Sugar Composition and Nitrogen Uptake of Two Yacon (Smallanthus sonchifolius Poepp. & Endl.) Genotypes
Previous Article in Special Issue
Progression of the Total and Individual Capsaicinoids Content in the Fruits of Three Different Cultivars of Capsicum chinense Jacq.
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Agronomy 2019, 9(3), 153; https://doi.org/10.3390/agronomy9030153

Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle

1
Institute of Sciences of Food Production, National Research Council, 10095 Grugliasco, Italy
2
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10-748 Olsztyn, Poland
3
Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy
*
Author to whom correspondence should be addressed.
Received: 5 March 2019 / Revised: 18 March 2019 / Accepted: 21 March 2019 / Published: 23 March 2019
  |  
PDF [599 KB, uploaded 23 March 2019]
  |  

Abstract

It is important to identify the growth stage at which the plant has the maximum antioxidant properties for the production of bioactive compounds from crops or agricultural by-products or for forage as a possible source of antioxidants in livestock. Therefore, we investigated the phenolic composition and antioxidant capacity of the aerial part of soybean at seven stages classified as vegetative stages (V5 and V6) and reproductive stages (R1, R2, R3, R4, and R5). Aqueous-methanol extracts were evaluated for their total phenolic content (TPC), ferric-reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC), antioxidant activity as determined by photochemiluminescence assay (PCL-ACL), Fe2+ chelating ability, and antiradical activity against DPPH. The extracts with the highest TPC content were obtained at stages V6 and R5. The phenolic compounds profile, as determined by DAD-HPLC, was characterized by 19 compounds, that differed significantly by growth stage (p < 0.05). Antioxidant tests showed significant differences among stages (p < 0.05). The lowest TEAC value was found for the R2 stage and the highest values for the R3 and R1 stages. FRAP values ranged from 623 to 780 μmol Fe2+/g extract. PCL-ACL values ranged from 516 to 560 μmol Trolox eq./g extract; Fe2+ chelation ability ranged from 36.5 to 51.7%. The highest antiradical activity against DPPH was found in the extract from the V5 stage, which had the lowest EC50 value. The extracts of soybean plant can be used in pharmacy for the production of nutraceuticals by virtue of their good antioxidant activity and content of flavonols and other bioactive constituents. View Full-Text
Keywords: Glycine max; morphological stage; antioxidant activity; phenolics Glycine max; morphological stage; antioxidant activity; phenolics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Peiretti, P.G.; Karamać, M.; Janiak, M.; Longato, E.; Meineri, G.; Amarowicz, R.; Gai, F. Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle. Agronomy 2019, 9, 153.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Agronomy EISSN 2073-4395 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top