During construction disturbance, topsoil is often removed and turfgrasses are established in poor soils. Our study determined the effects of amending subsoil with composted poultry litter on physical and chemical properties that affect turfgrass growth attributes. To simulate typical disturbance conditions, 20 cm of topsoil was removed from a Dormont silt loam (fine-loamy, mixed, superactive, mesic Oxyaquic Hapludalfs) and composted poultry litter was incorporated at 0.1, or 0.2, or 0.4 cm/cm-soil into the exposed subsoil to a depth of 12.7 cm before growing turf. Composted plots were compared to N-fertilized (50 × 10−4
) and control plots. Linear increases in total water content, organic matter, pH, and basic cations were observed following compost incorporation. Composted poultry litter increased total water content by 38% and decreased soil bulk density by 42%. Compost applications increased organic matter by 5.8%–6.4%, along with an increase in pH from 6.0–7.4. The cation exchange capacity increased up to 186% in compost-incorporated plots. No differences were observed between fertilized and control plots for all soil properties except for P levels, which increased in fertilized plots. Overall, compost treatments improved soil physical and chemical properties compared to conventionally fertilized and control plots.