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Effects of Pseudomonas fluorescens on the Water Parameters of Mycorrhizal and Non-Mycorrhizal Seedlings of Pinus halepensis
Departamento de Silvopascicultura. E.T.S.I. Montes and E.U.I.T. Forestal. Universidad Politécnica de Madrid Av/Ciudad Universitaria s/n. 28040, Madrid, Spain
Departamento de Protección Forestal. CIFOR-INIA (Instituto Nacional de Investigaciones y Tecnología Agrarias). Ctra. Coruña, Km. 7, 28040, Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 9 May 2013; in revised form: 2 August 2013 / Accepted: 2 August 2013 / Published: 20 August 2013
Abstract: Inoculation of forest seedlings with mycorrhizal fungi and rhizobacteria can improve the morphological and physiological qualities of plants, especially those used for regeneration of arid areas. In this paper, under standard nursery conditions, Aleppo pine seedlings were inoculated with Pseudomonas fluorescens CECT 5281 rhizobacteria. Some of these seedlings were also inoculated with the ectomycorrhizal fungus Pisolithus tinctorius. Five months after the inoculations, we examined the growth, water parameters (osmotic potential at full turgor [Ψπfull], osmotic potential at zero turgor [Ψπ0], and the tissue modulus of elasticity near full turgor [Emax]), mycorrhizal colonisation, and concentration of macronutrients (N, P, K, Ca and Mg) in the seedlings. Subsequently, a trial was conducted to assess the root growth potential. P. fluorescens CECT 5281 decreased the cellular osmotic potential of P. halepensis seedlings but increased its elasticity. P. tinctorius + P. fluorescens caused osmotic adjustment at zero turgor and increased tissue elasticity, which improved tolerance to water stress. All inoculations improved the growth and nutrition of the seedlings but caused non-significant effects on root growth potential. The co-inoculation Pisolithus tinctorius + Pseudomonas fluorescens at the nursery may be a suitable technique for producing improved seedling material for restoration purposes.
Keywords: rhizobacteria; osmotic adjustment; elastic adjustment; Pisolithus tinctorius; Pinus halepensis
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Domínguez-Núñez, J.A.; Muñóz, D.; de la Cruz, A.; Saiz de Omeñaca, J.A. Effects of Pseudomonas fluorescens on the Water Parameters of Mycorrhizal and Non-Mycorrhizal Seedlings of Pinus halepensis. Agronomy 2013, 3, 571-582.
Domínguez-Núñez JA, Muñóz D, de la Cruz A, Saiz de Omeñaca JA. Effects of Pseudomonas fluorescens on the Water Parameters of Mycorrhizal and Non-Mycorrhizal Seedlings of Pinus halepensis. Agronomy. 2013; 3(3):571-582.
Domínguez-Núñez, José A.; Muñóz, Daniel; de la Cruz, Ana; Saiz de Omeñaca, José A. 2013. "Effects of Pseudomonas fluorescens on the Water Parameters of Mycorrhizal and Non-Mycorrhizal Seedlings of Pinus halepensis." Agronomy 3, no. 3: 571-582.