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Agronomy 2013, 3(2), 474-487;

Glyphosate-Resistant Goosegrass from Mississippi

Crop Production Systems Research Unit, USDA-ARS, 141 Experiment Station Road, Stoneville, MS 38776, USA
Author to whom correspondence should be addressed.
Received: 6 May 2013 / Revised: 17 May 2013 / Accepted: 17 May 2013 / Published: 29 May 2013
(This article belongs to the Special Issue Weed Management and Herbicide Resistance)
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A suspected glyphosate-resistant goosegrass [Eleusine indica (L.) Gaertn.] population, found in Washington County, Mississippi, was studied to determine the level of resistance and whether the resistance was due to a point mutation, as was previously identified in a Malaysian population. Whole plant dose response assays indicated a two- to four-fold increase in resistance to glyphosate. Leaf disc bioassays based on a glyphosate-dependent increase in shikimate levels indicated a five- to eight-fold increase in resistance. Sequence comparisons of messenger RNA for epsps, the gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase, from resistant and sensitive goosegrass, revealed a cytosine to thymine nucleotide change at position 319 in the resistant accessions. This single nucleotide polymorphism causes a proline to serine amino acid substitution at position 106 in 5-enolpyruvylshikimate-3-phosphate synthase. A real-time polymerase chain reaction assay using DNA probes specific for the nucleotide change at position 319 was developed to detect this polymorphism. Goosegrass from 42 locations were screened, and the results indicated that glyphosate-resistant goosegrass remained localized to where it was discovered. Pendimethalin, s-metolachlor, clethodim, paraquat and fluazifop controlled resistant goosegrass 93% to 100%, indicating that several control options for glyphosate-resistant goosegrass are available. View Full-Text
Keywords: glyphosate resistance; point mutation; single nucleotide polymorphism; Eleusine indica glyphosate resistance; point mutation; single nucleotide polymorphism; Eleusine indica

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Molin, W.T.; Wright, A.A.; Nandula, V.K. Glyphosate-Resistant Goosegrass from Mississippi. Agronomy 2013, 3, 474-487.

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